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SAB4200086

Sigma-Aldrich

Anti-Rab32 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-RAS-associated protein RAB32

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 1-2 μg/mL using human HeLa cells
western blot: 1-2 μg/mL using whole extracts of human platelets

UniProt accession no.

Q13637

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAB32(10981)

General description

The gene RAB32 (Ras-related protein) is mapped to human chromosome 6q24.3. It is a Rab GTPase which is present in the mitochondria. The protein also has a transient phagosomal localization and is also present at the melanosome membrane.

Application

Anti-Rab32 antibody produced in rabbit has been used for western blotting and immunofluorescence.

Biochem/physiol Actions

RAB32 (Ras-related protein) controls intracellular lipid accumulation in hepatocytes. Absence of it promotes the expression of ATGL (adipose triglyceride lipase) and thereby causes lipolysis. RAB32 is involved in hepatic steatosis. In melanogenesis, it is involved in the transport of important enzymes, such as tyrosinase and Tyrp1 (tyrosinase related protein 1). RAB32 also interacts with and is involved in localization of leucine-rich repeat kinase 2 (LRRK2), a protein associated with Parkinson′s disease. RAB32 is hypermethylated in microsatellite-unstable colon, gastric and endometrial adenocarcinomas.
Rab32 facilitates mitochondrial anchoring of protein kinaseA (PKA) and mitochondrial dynamics. Rab32 was also found to be required for the formation of autophagic vacuoles.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Waschbusch D, et al.
<BIG>Rab32 interacts with SNX6 and affects retromer-dependent Golgi trafficking</BIG>
PLoS ONE, 14, e0208889-e0208889 (2019)
Jiaying Yu et al.
Frontiers in immunology, 11, 560110-560110 (2020-11-24)
HPS1, a BLOC-3 subunit that acts as a guanine nucleotide exchange factor of Rab32/38, may play a role in the removal of VAMP7 during the maturation of large dense core vesicles of Paneth cells. Loss of HPS1 impairs lysozyme secretion
A small GTPase, human Rab32, is required for the formation of autophagic vacuoles under basal conditions
Hirota Y, et al.
Cellular and Molecular Life Sciences, 66(17), 2913-2932 (2009)
Rab32 is an A-kinase anchoring protein and participates in mitochondrial dynamics. J Cell Biol 158: 659-668
Alto NM, et al.
The Journal of cell biology, 158(4), 659-668 (2002)
Functional analysis of novel Rab GTPases identified in the proteome of purified Legionella-containing vacuoles from macrophages.
Hoffmann C, et al.
Cellular Microbiology, 16, 1034-1052 (2014)
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