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SAB4200089

Sigma-Aldrich

Anti-DGCR8 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-DGCRK8, Anti-DiGeorge syndrom critical region 8

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~100 kDa

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 2.5-5 μg using lysates of HEK-293T cells over expressing human DGCR8
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human DGCR8
western blot: 1-2 μg/mL using lysates of HEK-293T cells over expressing human DGCR8

UniProt accession no.

Q8WYQ5

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DGCR8(54487)
mouse ... Dgcr8(94223)

Related Categories

General description

DGCR8 (DGCR8 microprocessor complex subunit) is a double-stranded RNA-binding protein mapped to the chromosome 22q11.2. It is composed of a WW domain and two double-stranded RNA-binding domains (dsRBDs).
DGCR8 contains an N-terminal region which is critical for nuclear localization and C-terminus which can directly and stably interact with the pri-mRNAs.

Application

Anti-DGCR8 (N-terminal) antibody produced in rabbit has been used in western blotting.
Anti-DGCR8 (N-terminal) antibody produced in rabbit has been used in:
  • western blotting
  • immunoprecipitation
  • immunofluorescence

Anti-DGCR8 (N-terminal) antibody produced in rabbit is suitable for immunoprecipitation (2.5-5μg using lysates of HEK-293T cells over expressing human DGCR8), indirect immunofluorescence (2-5μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human DGCR8) and western blot at a dilution of 1-2μg/mL (using lysates of HEK-293T cells over expressing human DGCR8).

Biochem/physiol Actions

DGCR8 (DGCR8 microprocessor complex subunit) participates in the biogenesis of microRNA (miRNA, miR) as a major component of the microprocessor complex. DGCR8 provides guidance to the RNase III enzyme, Drosha during rRNA processing. It forms the microprocessor complex by binding to the RNase III enzyme Drosha, which further converts long primary miRNAs (pri-miRNAs) into short hairpins called precursor miRNAs (pre-miRNAs). The processed hairpins finally are transported to the cytoplasm for further processing by Dicer into mature miRNAs. DGCR8 is also required for global gene regulation and silencing of embryonic stem cell self-renewal. Deletion of DGCR8 chromosomal location has been reported in the DiGeorge and velocardiofacial syndrome.
DGCR8, also known as DiGeorge syndrome critical region 8, DGCRK8 is the cofactor that interacts with drosha and forms a functional complex called the ‘‘Microprocessor” which is essential for microRNA (miRNA) maturation. DGCR8 contains an N-terminal region which is critical for nuclear localization and the C-terminal region stably interacts with the pri-miRNAs.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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A novel role for GSK3beta as a modulator of Drosha microprocessor activity and MicroRNA biogenesis
Fletcher CE, et al.
Nucleic Acids Research, 45(5) (2016)
DGCR8 is essential for microRNA biogenesis and silencing of embryonic stem cell self-renewal.
Nature Genetics, 39(3), 380-385 (2007)
Processing of primary microRNAs by the Microprocessor complex
Denli AM, et al.
Nature, 432(7014), 231-231 (2004)
Characterization of DGCR8/Pasha, the essential cofactor for Drosha in primary miRNA processing
Yeom KH, et al.
Nucleic Acids Research, 34(16) (2006)
Claire E Fletcher et al.
Nucleic acids research (2016-12-03)
Regulation of microRNA (miR) biogenesis is complex and stringently controlled. Here, we identify the kinase GSK3β as an important modulator of miR biogenesis at Microprocessor level. Repression of GSK3β activity reduces Drosha activity toward pri-miRs, leading to accumulation of unprocessed
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