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SAB4200112

Sigma-Aldrich

Anti-EIF2C3/Ago3 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-Argonaute 3, Anti-Eukaryotic translation initiation factor 2C, subunit 3

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~97 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 2.5-5 μg using lysates of HEK-293T cells over expressing human EIF2C3/Ago3
indirect immunofluorescence: 2.5-5 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human EIF2C3/Ago3
western blot: 0.5-1 μg/mL using lysates of HEK-293T cells over expressing human EIF2C3/Ago3

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EIF2C3(192669)

Related Categories

General description

Anti-eukaryotic translation initiation factor 2C 3 (EIF2C3)/protein argonaute-3 (Ago3) is developed in rabbit using as immunogen a synthetic peptide corresponding to human EIF2C3/Ago3 conjugated to KLH. Argonaute proteins (molecular mass 100 kDa) are characterized by piwi-argonaute-zwille (PAZ) and PIWI domains. Ago subfamily consists of four members, hsAgo1-4. hsAgo2 and Ago3 associate primarily with miRNA. Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies. A member of this group, Ago1, is associated with the Golgi and endoplasmic reticulum. The gene is located on chromosome 1 in a cluster of closely related family members including Ago 1 and Ago 4.
Argonaute 3, RISC catalytic component (Ago3), also known as eukaryotic translation initiation factor 2C, 3 (EIF2C3), is encoded by the gene mapped to human chromosome 1p34.3. The encoded protein belongs to the argonaute family of proteins and is characterized with a PAZ (Piwi/Argonaute/Zwille) domain and PIWI domains.

Immunogen

synthetic peptide corresponding to amino acids 13-25 of human EIF2C3/Ago3 conjugated to KLH. The corresponding sequence differs by one or two amino acid in rat and mouse, respectively.

Application

Anti-EIF2C3/Ago3 antibody produced in rabbit has been used in immunoprecipitation and western blot assays.
Anti-EIF2C3/Ago3 antibody produced in rabbit has been used:
  • immunoprecipitation
  • immunoblotting
  • immunofluorescence

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Argonaute proteins play a vital role in RNA interference (RNAi). These proteins are also involved in germline development, stem cell fate determination, as well as epigenetic regulation and transposon silencing. Ago3 facilitates the regulation of let-7a-3p expression. Mutation in the gene might lead to the development of Wilms′ tumors.
hAgo2 has been shown to have slicing activity. This region is lost in Wilms′ tumors, which are hypothesized to be caused by defects in embryonic kidney development, which disturb the capacity of metanephrogenic precursor cells to differentiate.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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[The functional analysis of polymorphic insertions of Alu retroelements at acute lymphoblastic leukemia].
Komkov Aiu
Bioorganicheskaia khimiia, 38, 351-364 (2012)
PAPI, a novel TUDOR-domain protein, complexes with AGO3, ME31B and TRAL in the nuage to silence transposition
Liu L
Development, 138, 1863-1873 (2011)
Hypoxia potentiates microRNA-mediated gene silencing through posttranslational modification of Argonaute2
Wu C, et al.
Molecular and Cellular Biology, 31(23), 4760-4774 (2011)
The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis
Carmell MA
Genes & Development, 16, 2733-2742 (2002)
eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference
Yi T
Nature Communications, 6 (2015)
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