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SAB4200257

Sigma-Aldrich

Anti-PSMA antibody, Mouse monoclonal

clone 107-1A4, purified from hybridoma cell culture

Synonym(s):

PSMA Antibody - Monoclonal Anti-PSMA antibody produced in mouse, Psma Antibody, Monoclonal Anti-FOLATE HYDROLASE 1, Monoclonal Anti-FOLH1, Monoclonal Anti-GCP2, Monoclonal Anti-GLUTAMATE CARBOXYPEPTIDASE II, Monoclonal Anti-N-ACETYLATED ALPHA-LINKED ACIDIC DIPEPTIDASE 1, Monoclonal Anti-NAALAD, Monoclonal Anti-PROSTATE-SPECIFIC MEMBRANE ANTIGEN, Monoclonal Anti-PSM

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

107-1A4, monoclonal

form

buffered aqueous solution

species reactivity

human

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 5-10 μg/mL using methanol/acetone fixed LNCaP cells.

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FOLH1(2346)

Related Categories

General description

Monoclonal Anti-PSMA (mouse IgG1 isotype) is derived from the hybridoma 107-1A4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Prostate-specific membrane antigen (PSMA) is an integral, non-shed, type 2 transmembrane glycoprotein belonging to the M28 peptidase family.
Prostate-specific membrane antigen (PSMA), also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1 (FOLH1), is a 100kDa membrane glycoprotein, composed of 750 amino acids. PSMA gene with 19 exons and 18 introns is mapped to human chromosome 11p11–12. The encoded protein is detected in various type of tissues including kidney, prostate and brain. PSMA contains short cytoplasmic amino terminus, a single membrane-spanning domain and a large extracellular domain.

Immunogen

prostate cancer LNCaP cells.

Application

Monoclonal Anti-PSMA antibody produced in mouse has been used in:
  • enzyme linked immuno sorbent assay (ELISA)
  • immunocytochemistry
  • immunoprecipitation
  • flow cytometry

Biochem/physiol Actions

Prostate-specific membrane antigen (PSMA), expressed in the brain, catalyzes the hydrolysis of N-acetyl-aspartyl glutamate to produce free glutamate. Elevated expression of the protein is associated with the development of prostate tumor. Mutations in PSMA leads to hyperhomocysteinemia. PSMA functions as a potential biomarker of prostate adenocarcinoma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Investigational antibody drug conjugates for solid tumors
Sapra P, et al.
Expert Opinion on Investigational Drugs, 20(8), 1131-1149 (2011)
Tumor target prostate specific membrane antigen (PSMA) and its regulation in prostate cancer.
Ghosh A and Heston WD
Journal of Cellular Biochemistry, 91, 528-539 (2004)
Construction of Prostate-Specific Expressed Recombinant Plasmids With High Transcriptional Activity of Prostate-Specific Membrane Antigen (PSMA) Promoter/Enhancer
Zeng H
Journal of Andrology, 26, 215-221 (2005)
Benedikt Kranzbühler et al.
The Prostate, 79(12), 1450-1456 (2019-06-25)
Prostate-specific membrane antigen (PSMA)-based imaging and therapy are increasingly used in the management of prostate cancer. However, low PSMA surface expression in certain patients is a limitation for PSMA-based technologies. We have previously shown that high doses of dutasteride, a
Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II.
Barinka C
Journal of Neurochemistry, 80, 477-487 (2002)
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