SAB4200338
Anti-KLF5 antibody , Mouse monoclonal
clone KLF5-55, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-BTEB2, Monoclonal Anti-CKLF, Monoclonal Anti-IKLF, Monoclonal Anti-KLF5, Monoclonal Anti-Kruppel-like factor 5 (intestinal)
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All Photos(3)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
KLF5-55, monoclonal
form
buffered aqueous solution
mol wt
antigen ~55
species reactivity
rat, mouse, chicken, human, hamster, canine, monkey
concentration
~1.0 mg/mL
technique(s)
indirect immunofluorescence: 2.5-5.0 μg/mL using methanol/acetone fixed PC3 cells.
western blot: 1.0-2.0 μg/mL using extracts of PC3 cells.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... KLF5(688)
mouse ... Klf5(12224)
rat ... Klf5(84410)
General description
Kruppel like factor 5 (KLF5) is encoded by the gene mapped to human chromosome 13q22.1. The encoded protein belongs to kruppel-like factor (KLF) protein family and is mainly expressed in variety of tissues, including lung, colon, intestine and pancreas.
Monoclonal Anti-KLF5 (mouse IgG1 isotype) is derived from the hybridoma KLF-55 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. KLF5 is a DNA-binding transcriptional regulator. This protein contains three independent peptide modules of the C2H2 zinc finger-type. KLF5 protein is expressed in the prostate, kidney, skeletal muscle, and breast. KLF5 gene is also expressed in embryos and is highly expressed in the later stage of fetal development.
Specificity
Monoclonal Anti-KLF5 recognizes human, monkey, dog, hamster, mouse and rat KLF5.
Immunogen
synthetic peptide corresponding to a sequence at the C-terminus of human KLF5, conjugated to KLH. The corresponding sequence is identical to monkey, bovine, chicken, rat and mouse KLF5.
Application
Anti-KLF5 antibody, Mouse monoclonal has been used in western blotting and immunofluorescence.
Biochem/physiol Actions
KLF5 protein functions through the restoration of cyclin D1, cyclin B1 and Cdc2 gene expression. This protein is also involved in regulating the inhibitory effect of retinoids on cell proliferation. KLF5 protein regulates the pro-proliferative and transforming activities of oncogenic H-ras. KLF5 protein plays a role as a tumor suppressor in intestinal tumorigenesis in vivo. Deletion and down-regulation of KLF5 leads to prostate and breast cancer.
Kruppel like factor 5 (KLF5) has been involved in the regulation of various cellular functions, such as cell cycle, apoptosis, proliferation and differentiation, in both normal and tumor cells. Additionally, it also plays a vital role in maintenance of cardiovascular remodeling. Overexpression of the gene has been observed in various cancer types, including cervical cancer(CC) and colon cancer. Hence, this can be used as a prognostic marker for these cancers.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Dan Tong et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 12(8), 2442-2448 (2006-04-28)
Kruppel-like factor (KLF5) is a cell growth mediator in various epithelial cells. Higher KLF5 increases cell growth rate and leads to transformed phenotypes. Because tumor cell proliferation is tightly associated with tumor progression, and consequently, with survival of cancer patients
Characterization of the expression profiles of adipogenesis-related factors, ZNF423, KLFs and FGF10, during preadipocyte differentiation and abdominal adipose tissue development in chickens
Matsubara Y
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 165, 189-195 (2013)
KLF5 promotes hypoxia-induced survival and inhibits apoptosis in non-small cell lung cancer cells via HIF-1?
Li X
International Journal of Oncology, 45, 1507-1514 (2014)
FBW7-mediated ubiquitination and degradation of KLF5
Yi Luan
World Journal of Biological Chemistry, 5, 216-223 (2014)
Alejandro D Hofmann et al.
Pediatric surgery international, 30(12), 1191-1197 (2014-10-18)
The high morbidity and mortality in congenital diaphragmatic hernia (CDH) is attributed to pulmonary hypoplasia and persistent pulmonary hypertension (PH). PH is characterized by increased pulmonary artery smooth muscle cell (SMC) proliferation, suppressed apoptosis as well as endothelial dysfunction. Krüppel-like
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