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SAB4200346

Sigma-Aldrich

Anti-SMAD7 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, suitable for western blot: 2 μg/mL

Synonym(s):

Anti-CRCS3, Anti-MAD homolog 7, Anti-MAD homolog 8, Anti-MADH7, Anti-MADH8, Anti-Mothers against DPP homolog 7, Anti-Mothers against DPP homolog 8, Anti-SMAD

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~46 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 1-2 μg/mL using human A549 cells.
western blot: 2-4 μg/mL using whole extracts of HEK-293 cells over-expressing human SMAD7.

UniProt accession no.

O15105

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SMAD7(4092)

Related Categories

General description

SMAD7 (mothers against decapentaplegic homolog 7) is a Tbx1 (T-box 1) interacting gene and is encoded by the gene mapped to human chromosome 18q21.1.
SMAD7 (mothers against decapentaplegic homolog 7), that binds the E3 ubiquitin ligase Smurf1/2. SMAD7 belongs to the family of inhibitory Smads (I-Smads).

Immunogen

peptide corresponding to the N-terminal region of human SMAD7, conjugated to KLH. The corresponding sequence is identical in monkey and differs by 2 amino acids in mouse and rat.

Application

Anti-SMAD7 (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

SMAD7 (mothers against decapentaplegic homolog 7) is a modulator of TGFβ (transforming growth factor β) signaling in immune cells which are associated with ulcerative colitis and inflammatory bowel syndrome. It plays a major role in the etiology of CRC (colorectal cancer). In addition, it also acts as a mediator of the negative feedback loop for both the TGFβ and BMP (bone morphogenetic proteins) signaling pathways. It is essential for the remodelling of pharyngeal artery and the enlargement of great vessel. In mouse, homozygous removal of Smad7 causes primarily fourth-related arch artery defects. Silencing of Smad7 in RCD (refractory coeliac disease) biopsy samples can decrease the expression of interleukin-6 and tumour necrosis factor-α. Polymorphism of this gene is associated with colorectal cancer. SMAD7 is an inhibitor of the TGFβ (transforming growth factor beta) /BMP (bone morphogenetic proteins) pathway.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Smad7: not only a regulator, but also a cross-talk mediator of TGF-beta signalling
Yan X and Chen YG
The Biochemical Journal, 434(1), 1-10 (2011)
Multiple functional risk variants in a SMAD7 enhancer implicate a colorectal cancer risk haplotype.
Fortini BK
PLoS ONE, 9 (2014)
Ning Sun et al.
EBioMedicine, 62, 103108-103108 (2020-11-14)
Hepatocellular carcinoma (HCC) is a leading cause of cancer death worldwide, with unmet need for the pharmacological therapy. The functions of ATXN7L3 in HCC progression are not known. RNA sequence, quantitative real-time PCR, and western blot were performed to detect
High Smad7 sustains inflammatory cytokine response in refractory coeliac disease.
Sedda S
Immunology, 150, 356-363 (2017)
Intronic polymorphisms of the SMAD7 gene in association with colorectal cancer.
Damavand B
Asian Pacific Journal of Cancer Prevention, 16, 41-44 (2015)
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