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SAB4200539

Sigma-Aldrich

Anti-Myocardin antibody produced in rabbit

enhanced validation

affinity isolated antibody

Synonym(s):

Anti-MYCD, Anti-MYOCD

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~107 kDa

species reactivity

rat, human, mouse

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunohistochemistry: 5-10 μg/mL using human and chicken heart.
indirect immunofluorescence: 2-4 μg/mL using A10 cells.
western blot: 1.5-3.0 μg/mL using extracts of Hct116 cells

UniProt accession no.

Q8IZQ8

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYOCD(93649)
mouse ... Myocd(214384)
rat ... Myocd(246297)

Related Categories

General description

Anti-Myocardin specifically recognizes human, rat and chicken myocardin (MYOCD). Myocardin is encoded by the gene mapped to human chromosome 17p11.2. It contains a conserved SAP (SAF-A/B, Acinus, and PIAS) domain, involved in the regulation of nuclear organization, chromosomal dynamics and apoptosis. The encoded protein is expressed in a precise developmentally regulated, lineage-restricted pattern in the embryo and during postnatal development.

Immunogen

a synthetic peptide corresponding to an internal sequence of human myocardin, conjugated to KLH. The corresponding sequence is identical in human myocardin isoform 2 and in rat and mouse myocardin.

Biochem/physiol Actions

Myocardin (MYOCD) acts as an important smooth and cardiac muscle-specific transcriptional coactivator of serum response factor (SRF). It also plays an essential role in smooth muscle cell (SMC) differentiation. The encoded protein regulates bone morphogenetic protein 10 (BMP10) expression and is required for cardiac development, as well as cardiomyocyte survival and maintenance of heart function. MYOCD expression is associated with the development of atherosclerosis, Alzheimer′s disease and malignant transformation.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sofie De Moudt et al.
Physiological reports, 10(18), e15410-e15410 (2022-09-20)
In vivo angiotensin II (AngII)-treatment is a widely used experimental model to induce cardiovascular disease and results in a high likelihood of abdominal aorta aneurysm (AAA) formation. This involves progressive and irreversible focal dilation of the abdominal aorta and induces
Myocardin-related transcription factors: critical coactivators regulating cardiovascular development and adaptation.
Parmacek MS
Circulation Research, 100, 633-644 (2007)
Loss of heterozygosity: what is it good for?
Ryland GL, et al.
BMC Medical Genomics (2015)
Myocardin regulates BMP10 expression and is required for heart development
Huang J, et al.
The Journal of Clinical Investigation, 122, 3678-3691 (2012)
Jiantong Hou et al.
Experimental cell research, 382(2), 111476-111476 (2019-07-01)
The phenotypic transition of pulmonary artery smooth muscle cells (PASMCs) from a contractile/differentiated to synthetic/de-differentiated phenotype is an important mechanism for the occurrence and development of hypoxic pulmonary hypertension (HPH). Integrin-linked kinase (ILK) is an early hypoxic response factor whose
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