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SAB4200598

Sigma-Aldrich

Monoclonal Anti-FTH1 antibody produced in mouse

~1.0 mg/mL, clone FTH24, purified immunoglobulin

Synonym(s):

Monoclonal Anti-FHC, Monoclonal Anti-FTH, Monoclonal Anti-FTH1, Monoclonal Anti-FTHL6, Monoclonal Anti-OK/SW-cl.84, Monoclonal Anti-PIG15, Monoclonal Anti-PLIF, Monoclonal Anti-cell proliferation-inducing gene 15 protein, Monoclonal Anti-ferritin H subunit, Monoclonal Anti-ferritin heavy chain, Monoclonal Anti-ferritin, heavy polypeptide 1, Monoclonal Anti-placenta immunoregulatory factor, Monoclonal Anti-proliferation-inducing protein 15

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FTH24, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

flow cytometry: 10-20 μg/test using HeLa cells
immunoblotting: 0.5-1.0 μg/mL using total cell extracts of HEK-293T cells overexpressing FTH1 (expected: ~26kDa).
indirect immunofluorescence: 5-10 μg/mL using HeLa cells

isotype

IgG1

UniProt accession no.

P02794

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FTH1(2495)

Related Categories

General description

Monoclonal Anti-FTH1 (mouse IgG1 isotype) is derived from the hybridoma FTH24 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal sequence of human FTH1. Ferritin, a highly conserved iron-binding protein, is composed of two subunits, namely a 21 kDa heavy (H) chain and a 19 kDa light (L) chain. Ferritin heavy chain 1 gene is mapped on human chromosome 11q13. It is mainly found in heart and pancreas.

Specificity

Monoclonal Anti- FTH1 recognizes human FTH1.

Immunogen

synthetic peptide corresponding to an internal sequence of human FTH1. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Application

Monoclonal Anti-FTH1 antibody produced in mouse may be used in:
  • immunoblotting
  • immunocytochemistry
  • flow cytometry
  • immunofluorescence

Biochem/physiol Actions

Ferritin heavy chain catalyzes the first step in iron storage, the oxidation of Fe(II) to Fe(III). In addition to iron buffering, heavy chain ferritin also enhances de novo thymidine biosynthesis. Serum ferritin level is the most commonly used marker for determining iron status stored in the body. It is markedly influenced by factors such as inflammation, malnutrition, malignancy, and iron overload disorders. Studies have found that defects in ferritin proteins are also associated with several neurodegenerative diseases. FTH1 can also be used as a possible prognostic marker for breast cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Effects of pulsed electromagnetic fields on human osteoblastlike cells (MG-63): a pilot study.
Sollazzo V, et al.
Clinical Orthopaedics and Related Research, 468(8), 2260-2277 (2010)

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