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SAB4200661

Sigma-Aldrich

Monoclonal Anti-Dynamin 2 (DNM2) antibody produced in mouse

clone DYN2-11, purified from hybridoma cell culture

Synonym(s):

CMT2M, CMTDI1, CMTDIB, DI-CMTB, DYN2, DYNII, LCCS5, dynamin 2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DYN2-11

form

buffered aqueous solution

mol wt

antigen ~98 kDa

species reactivity

mouse, human, monkey, canine, rat

concentration

~1 mg/mL

technique(s)

immunoblotting: 2-4 μg/mL using using whole extract of HeLa cells.
immunofluorescence: 5-10 μg/mL using using HeLa cells.
immunoprecipitation (IP): 5-10 μg using using whole extract of HeLa cells.

isotype

IgG1

UniProt accession no.

P50570

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DNM2(1785)
mouse ... Dnm2(13430)
rat ... Dnm2(25751)

Related Categories

General description

Monoclonal Anti-Dynamin 2 (DNM2) (mouse IgG1 isotype) is derived from the hybridoma DYN2-11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Dynamin 2 (DNM2) also known as DYN2 belongs to the dynamins family of guanosine triphosphatase (GTPase) proteins.
The gene DNM2 (dynamin 2) is mapped to human chromosome 19p13. It is widely expressed. The protein has a catalytic amino-terminal GTPase domain, a middle domain, a pleckstrin homology domain (PH), a GTPase effector domain (GED) and a less conserved carboxyl-terminal proline/arginine rich domain (PRD).

Immunogen

synthetic peptide corresponding to a sequence at the N-terminal region of human DNM2 , conjugated to KLH

Application

Monoclonal Anti-Dynamin 2 (DNM2) antibody produced in mouse has been used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Biochem/physiol Actions

DNM2 (dynamin 2) is a GTPase which is required for clathrin-mediated endocytosis. It is important for clathrin-coated pit maturation and clathrin-coated vesicle formation. DNM2 participates in neuronal morphology, axonal growth, centrosome cohesion, actin- and microtubular organization. Mutations in it are associated with Charcot-Marie-Tooth disease, centronuclear myopathy ADCNM (autosomal dominant centronuclear myopathy) and lethal congenital contractures syndrome type 5 (LCCS5). It is upregulated in prostate cancer.
Furthermore, DNM2 is upregulated in pancreatic cancer as it activates Ras-related C3 botulinum toxin substrate 1 (Rac1). It also promotes invasive cellular migration and lamellipod protrusion.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Dynamins at a glance.
Jürgen A W Heymann et al.
Journal of cell science, 122(Pt 19), 3427-3431 (2009-09-18)
Arlek M González-Jamett et al.
Frontiers in endocrinology, 4, 126-126 (2013-09-26)
Dynamin-2 is a ubiquitously expressed mechano-GTPase involved in different stages of the secretory pathway. Its most well-known function relates to the scission of nascent vesicles from the plasma membrane during endocytosis; however, it also participates in the formation of new
Multiple Dynamin family members collaborate to drive mitochondrial division
Lee JE, et al.
Nature, 540(7631), 139-139 (2016)
Shawn M Ferguson et al.
Nature reviews. Molecular cell biology, 13(2), 75-88 (2012-01-12)
Dynamin, the founding member of a family of dynamin-like proteins (DLPs) implicated in membrane remodelling, has a critical role in endocytic membrane fission events. The use of complementary approaches, including live-cell imaging, cell-free studies, X-ray crystallography and genetic studies in
Bin Xu et al.
Cancer medicine, 3(1), 14-24 (2014-01-10)
Dynamin 2 (Dyn2) is essential for intracellular vesicle formation and trafficking, cytokinesis, and receptor endocytosis. In this study, we investigated the implication of Dyn2 as a prognostic marker and therapeutic target for progressive prostate cancer (PCA). We evaluated Dyn2 protein
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