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SAB4200680

Sigma-Aldrich

Anti-Biotin antibody, Mouse monoclonal

clone BN-34, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Biotin antibody produced in mouse, Bios II, Coenzyme R, D-Biotin, Vitamin B7, Vitamin H

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

BN-34, monoclonal

form

buffered aqueous solution

species reactivity

canine, human, bovine, rat, mouse

concentration

~1 mg/mL

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: 0.15-0.3 μg/mL
western blot: suitable

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Biotin is a bi-heterocyclic, essential water soluble vitamin and a cofactor synthesized in most of the plants and prokaryotes. Protein-bound biotin in usually found in dietary sources like meat, cereals, egg yolk, liver and some vegetables.

Immunogen

biotinylated KLH

Biochem/physiol Actions

Biotin functions as a coenzyme for acetyl CoA, pyruvate, propionyl-CoA and 3-methylcrotonyl-CoA carboxylases for the regulation of gluconeogenesis, lipid homeostasis, pleotropic gene expression and to replenish the intermediates of tricarboxylic cycle. Biotin bound to histones plays an important role in chromatin remodeling, DNA repair and repression of transposons to maintain genome stability. Inadequate biotin levels may lead to dermatitis, conjunctivitis, alopecia, and central nervous system abnormalities.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Molecular biology of biotin attachment to proteins
Chapman-Smith Anne and Cronan Jr JE
The Journal of Nutrition, 129(2), 477S-484S (1999)
Biotin and biotinidase deficiency
Zempleni J, et al.
Expert Review of Endocrinology & Metabolism, 3(6) (2008)
Essentials of Biochemistry for Medical Students (2011)
Dietary Reference Intakes for Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline (1998)
Biotin: a timeless challenge for total synthesis
De Clercq PJ
Chemical Reviews, 97(6), 1755-1792 (1997)
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