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SAB4200706

Sigma-Aldrich

Anti-TM4SF4 antibody, Mouse monoclonal

clone TM4SF-11, hybridoma cell culture supernatant

Synonym(s):

Anti-ILTMP, Anti-il-TMP transmembrane 4 L six family member 4

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

antibody form

culture supernatant

Quality Level

200

antibody product type

primary antibodies

clone

TM4SF-11, monoclonal

form

buffered aqueous solution

mol wt

antigen ~27 kDa

species reactivity

rat, human, canine, monkey, mouse, bovine

technique(s)

immunoblotting: 1:4,000-1:8,000 using whole extract of HCT-116 cells

isotype

IgM

UniProt accession no.

P48230

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TM4SF4(7104)
mouse ... Tm4sf4(229302)
rat ... Tm4sf4(116467)

General description

Anti-TM4SF4 antibody, Mouse monoclonal (mouse IgM isotype) is derived from the TM4SF-11 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with synthetic peptide from the internal region of human TM4SF4, conjugated to KLH. TM4SF4 (Transmembrane 4 L6 Family member 4) also known as ILTMP (intestine and liver tetraspan membrane protein) is a member of the transmembrane 4 superfamily (or tetraspanin family). It is a cell surface glycoprotein containing four transmembrane domains.

Immunogen

synthetic peptide from the internal region of human TM4SF4

Application

Anti-TM4SF4 antibody has been used in immunoblotting.

Biochem/physiol Actions

TM4SF4 (Transmembrane 4 L6 Family member 4) can intervene density-dependent cell proliferation. TM4SF4 levels increases in non-dividing epithelial cells during their differentiation and migration of intestinal crypts. TM4SF4 levels are upregulated in 80% of hepatocellular carcinoma (HCC) tissues and in stomach adenocarcinoma cells. Reduction of TM4SF4 expression in HCC cell lines by stably transfecting TM4SF4 antisense plasmid caused significant inhibition of cell proliferation.

Physical form

The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Human tetraspanin transmembrane 4 superfamily member 4 or intestinal and liver tetraspan membrane protein is overexpressed in hepatocellular carcinoma and accelerates tumor cell growth
Li Y, et al.
Acta biochimica et biophysica Sinica, 44(3), 224-232 (2012)
Soo-Im Choi et al.
Oncotarget, 5(20), 9823-9837 (2014-10-27)
Transmembrane 4 L six family member 4 (TM4SF4) is a member of the tetraspanin L6 domain family. Other members of this family, TM4SF1 (also known as L6-Ag) and TM4SF5, have been shown to be upregulated in multiple tumors and involved
B M Wice et al.
The Journal of biological chemistry, 270(37), 21907-21918 (1995-09-15)
The human cell line HT-29 provides a model system for studying regulation of proliferation and differentiation in intestinal epithelial cell lineages: (i) HT-29 cells cultured in glucose resemble undifferentiated multipotent transit cells located in the lower half of intestinal crypts;
Ying Li et al.
Acta biochimica et biophysica Sinica, 44(3), 224-232 (2012-01-13)
The human transmembrane 4 superfamily member 4 or intestinal and liver tetraspan membrane protein (TM4SF4/il-TMP) was originally cloned as an intestinal and liver tetraspan membrane protein and mediates density-dependent cell proliferation. The rat homolog of TM4SF4 was found to be

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