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SAB4200805

Sigma-Aldrich

Anti-β-Galactosidase antibody, Mouse monoclonal

clone GAL-13, purified from hybridoma cell culture

Synonym(s):

Anti-Beta-gal, Anti-Beta-galactosidase, Anti-Lactase

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About This Item

UNSPSC Code:
12352203

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

GAL-13, monoclonal

description

Research area: Microbiome

form

buffered aqueous solution

species reactivity

E. coli

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable
indirect ELISA: 0.5-1 μg/mL using anti-Mouse antibody for coating, primary Anti-Beta-GAL antibody followed by addition of β-d-galactosidase enzyme from E. coli and 2-Nitrophenyl β-D-galactopyranoside as substrate.

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

Escherichia coli K12 ... Lacz(945006)

Related Categories

General description

Anti-nitric oxide synthase, inducible antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the NOS-IN hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a synthetic peptide corresponding to the C terminal region of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS), conjugated to KLH
Beta galactosidases have been obtained from microorganisms such as fungi, bacteria and yeasts; plants, animals cells and from recombinant sources. This enzyme has two main applications; the removal of lactose from milk products for lactose intolerant people and the production of galactosylated products.Monoclonal Anti-Beta-Galactosidase recognizes soluble Beta-d-galactosidase and retains the enzymatic activity. The antibody reacts with the native enzyme and does not recognize denatured or reduced Beta-galactosidase. The Anti-Beta-Galactosidase antibody maybe useful tool for immunoenzymatic staining of blood and bone marrow smears or tissue sections. In addition, this product may be used as a primary antibody for the detection and purification of recombinant fusion hybrid proteins, which contain Beta-galactosidase (lacZ) in the cloning vector.

Immunogen

Beta-D-Galactosidase purified from E. coli

Application

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal has been used in: immunoblotting immunofluorescence fluorescence-activated cell sorting 3 (FACS3) immunohistochemistry
The antibody is recommended to use in various immunological techniques, including ELISA, Immunofluorescence and immunohistology.

Biochem/physiol Actions

NO is a messenger molecule mediating diverse functions including vasodilatation, neurotransmission, antimicrobial and anti-tumor activities. It is implicated as a pathogenic mediator in a variety of conditions, including central nervous system (CNS) disease states, such as the animal model of multiple sclerosis (MS) and experimental allergic encephalomyelitis.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Erythropoietin-?-D-galactosidase the generation, purification and use of a fusion protein
Nielsen, O.J. et al.,
Journal of Immunological Methods, 111, 1-9 (1988)
Nitric oxide release: Part II
Carpenter AW and Mark HS
Chemical Society Reviews, 41(10), 3742-3752 (2012)
Neuronal nitric oxide synthase: structure, subcellular localization, regulation, and clinical implications
Zhou L and Zhu DY
Nitric Oxide, 20(4), 223-230 (2009)
Anjali Bajpai et al.
Developmental biology, 457(1), 1-8 (2019-09-27)
The Hedgehog (Hh) morphogen regulates growth and patterning. Since Hh signaling is also implicated in carcinogenesis, it is conceivable that de novo Hh-secreting organizers, if formed in association with oncogenic hit could be tumor-cooperative. Here we validate this hypothesis using
S Gillard-Blaas et al.
Journal of immunological methods, 139(2), 207-215 (1991-06-03)
A T cell hybridoma specific for a protein of Plasmodium chabaudi chabaudi has been used to test a system for direct T cell screening of a cDNA library of P. chabaudi in the phage lambda gt11. The technique is based
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