SAB4200806
Anti-Ezrin antibody, Mouse monoclonal
clone 3C12, purified from hybridoma cell culture
Synonym(s):
Cytovillin, Villin-2, p81
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
3C12, monoclonal
form
buffered aqueous solution
mol wt
~80 kDa
species reactivity
bovine, canine, hamster, kangaroo, human, monkey, rat, mouse
packaging
antibody small pack of 25 μL
concentration
~1 mg/mL
technique(s)
immunoblotting: 1-2 μg/mL using using canine MDCK cells extract
immunofluorescence: 0.5-1 μg/mL using human epidermoid carcinoma A431 cells.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Ezr(22350)
General description
Ezrin, also known as cytovillin, p81 or 80K, is a.member of the ERM proteins family composed of Ezrin, Radixin, and Moesin. These proteins are known as actin-binding proteins that link the cell membrane to the actin cytoskeleton, particularly in microvillus-bearing epithelial cells. Ezrin is present in microvilli, microspikes, and membrane ruffles of cells.
The ERM proteins family are involved in the functional expression of membrane proteins on the cell surface, integrate Rho guanosine 5′-triphosphatase (GTPase) signaling regulating cytoskeletal organization and act as protein kinase A (PKA)-anchoring proteins. Therefore, ERM proteins seem to play an important role in the membrane transport of electrolytes by ion channels and transporters.
The ERM proteins family are involved in the functional expression of membrane proteins on the cell surface, integrate Rho guanosine 5′-triphosphatase (GTPase) signaling regulating cytoskeletal organization and act as protein kinase A (PKA)-anchoring proteins. Therefore, ERM proteins seem to play an important role in the membrane transport of electrolytes by ion channels and transporters.
Immunogen
The carboxy-terminal part of recombinant human ezrin
Application
Monoclonal Anti-Ezrin recognizes an epitope located on the C-terminal region of Ezrin. Reactivity has been observed with human, mouse, hamster, monkey, bovine, canine, rat and rat-kangaroo origin. The antibody is recommended to use in various immunological techniques, including Immunoblotting (~80kDa), immunofluorescence, Immunohistochemistry and immunoprecipitation.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Other Notes
This product is for R&D use only, not for drug, household, or other uses.
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Kotoku Kawaguchi et al.
Biological & pharmaceutical bulletin, 40(4), 381-390 (2017-04-07)
Ezrin/radixin/moesin (ERM) proteins function as general cross-linkers between plasma membrane proteins and the actin cytoskeleton and are involved in the functional expression of membrane proteins on the cell surface. They also integrate Rho guanosine 5'-triphosphatase (GTPase) signaling to regulate cytoskeletal
A Bretscher
The Journal of cell biology, 97(2), 425-432 (1983-08-01)
The microvillus cytoskeleton, isolated from chicken intestinal epithelial cell brush borders, is known to contain five major protein components, the 110,000-dalton polypeptide, villin (95,000 daltons), fimbrin (68,000 daltons), actin (43,000 daltons), and calmodulin (17,000 daltons). In this paper we describe
Q Yang et al.
Proceedings of the National Academy of Sciences of the United States of America, 88(14), 5949-5953 (1991-07-15)
The polymerase chain reaction (PCR), from primers corresponding to conserved sequences within the catalytic domains of the protein-tyrosine phosphatases, was used to amplify protein-tyrosine phosphatase-related cDNAs from a HeLa cell library. After probing the same cDNA library with one of
K D Geiger et al.
The American journal of pathology, 157(6), 1785-1793 (2000-12-07)
The actin-binding protein ezrin has been associated with motility and invasive behavior of malignant cells. To assess the presence of this protein in human glial cells of the brain and its potential role in benign and malignant glial tumors, we
T Böhling et al.
The American journal of pathology, 148(2), 367-373 (1996-02-01)
Ezrin is a cytoskeleton-associated protein that appears to link actin filaments to the plasma membrane. Immunocytochemical studies suggest that ezrin is expressed in epithelial cells but not in mesenchymal cells. In addition, ezrin is expressed by certain epithelial tumors, such
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