SAB4200880
Anti-Cellular Fibronectin antibody, Mouse monoclonal
clone FN-3E2, purified from hybridoma cell culture
Synonym(s):
cFN
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
antibody form
purified from hybridoma cell culture
Quality Level
clone
FN-3E2
form
liquid
species reactivity
mouse, rat, human, monkey, chicken
concentration
~1 mg/mL
isotype
IgM
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Fibronectin is an extracellular matrix protein composed of two nearly identical disulfide-bound polypeptides with typical molecular weights of 220 - 240 kDa.1 Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma.
Specificity
Monoclonal Anti-Cellular Fibronectin labels fibronectin in the pericellular extracellular matrix of cultured chicken fibroblasts. It also labels cellular fibronectin in chicken embryo, mouse and human fibroblasts grown in tissue culture using in indirect immunofluorescent labeling technique.
Application
The antibody may also be used in immunoperoxidase or immunofluorescent labeling on frozen human tissue preparations. In immunoblotting, Monoclonal Anti-Cellular Fibronectin localizes the 240 kDa bands of cellular fibronectin in both its native and denatured forms.
Biochem/physiol Actions
Analysis of the fibronectin molecule indicates that it contains several functionally and structurally distinct domains, which may bind to cell surfaces, collagen, heparin, etc.2,3,4,5. Numerous studies have shown the fibronectin may enhance cell adhesion and spreading and affect the routes of cell migration both in vivo and in culture.6 It has been shown that upon malignant transformation many cells lose their surface bound fibronectin.7
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours. Protect from prolonged exposure to light.
Disclaimer
This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
wgk_germany
WGK 1
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