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SAB4500309

Sigma-Aldrich

Anti-Histone H4 (Acetyl-Lys12) antibody produced in rabbit

affinity isolated antibody

Synonym(s):

H4F2, HIST, HIST1H4A, Histone H4

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 11 kDa

species reactivity

human, rat, mouse

concentration

~1 mg/mL

technique(s)

ELISA: 1:1000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

P62805

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

acetylation (Lys12)

Gene Information

human ... H4-16(121504)

Related Categories

General description

Anti-Histone H4 (Acetyl-Lys12) Antibody detects endogenous levels of total Histone H4 (Acetyl-Lys12) protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys12.

Immunogen Range: 10-59

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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flash_point_f

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flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Maaike Waasdorp et al.
Scientific reports, 6, 33030-33030 (2016-09-14)
Endogenously administered activated protein C ameliorates diabetic nephropathy (DN) in a protease-activated receptor-1 (PAR-1)-dependent manner, suggesting that PAR-1 activation limits the progression of DN. Activation of PAR-1 in fibroblast-like cells, however, induces proliferation and extracellular matrix production, thereby driving fibrotic
Shuntaro Yamashita et al.
PloS one, 9(7), e101864-e101864 (2014-07-08)
In our previous studies, we reported that SIRT1 prevents cellular senescence in human fibroblast, and that SIRT1-induced inhibition of cellular senescence is due to enhanced hTERT gene expression. In this study, we investigate the molecular mechanisms behind SIRT1-induced potentiation of
Bo Xia et al.
Toxicology letters, 228(3), 241-247 (2014-05-27)
Chromium is a potent human mutagen and carcinogen. The capability of chromium to cause cancers has been known for more than a century, and numerous epidemiological studies have been performed to determine its carcinogenicity. In the post-genome era, cancer has

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