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SAB4502019

Sigma-Aldrich

Anti-NOTCH1 (Cleaved-Val1754) antibody produced in rabbit

affinity isolated antibody

Synonym(s):

NOTC1, Notch homolog 1, neurogenic locus notch 1, notch 1 (translocation-associated), translocation-associated (Drosophila) (TAN1)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 110 kDa

species reactivity

rat, mouse, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:5000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

P46531

UniProt accession no.

P46531

application(s)

research pathology

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

proteolytically cleaved (Val1754)

Gene Information

human ... NOTCH1(4851)

Related Categories

General description

The NOTCH1 gene is mapped to human chromosome 9q34 and encodes a member of the NOTCH family of proteins. The gene encodes a large transmembrane protein that contains an extracellular domain with 36 tandem epidermal growth factor (EGF)-like repeats and three cysteine-rich Notch/LIN-12 repeats, a six ankyrin repeat-containing intracellular domain, and a transactivation domain.

Anti-NOTCH1 (Cleaved-Val1754) Antibody detects endogenous levels of fragment of activated NOTCH1 (Cleaved-Val1754) protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human Notch 1.

Immunogen Range: 1735-1784

Application

Anti-NOTCH1 (Cleaved-Val1754) antibody produced in rabbit has been used in immunohistochemistry (IHC).

Biochem/physiol Actions

The Notch1 protein is a transmembrane receptor that induces pluripotent progenitor cells to form T-cells and facilitates the assembly of pre–T cell receptor complexes in immature thymocytes. It participates in an intracellular signaling pathway that regulates cellular differentiation, cell fate and lateral inhibition. Mutations in this gene have been associated with human blood cancers, abnormal cardiovascular calcification, aortic valve disease, and human T cell acute lymphoblastic leukemia.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Notch 1 is a valuable therapeutic target against cell survival and proliferation in clear cell renal cell carcinoma.
Zhuang Z, et al.
Oncology Letters, 14(3), 3437-3444 (2017)
Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia.
Weng A P, et al.
Science, 306(5694), 269-271 (2004)
Mutations in NOTCH1 cause aortic valve disease.
Garg V, et al.
Nature, 437(7056), 270-270 (2005)
Yan Li et al.
Bioengineered, 12(2), 10183-10193 (2021-12-08)
As the second most common cancer among women, cervical cancer is a huge threat to their health all over the world. Integral membrane protein 2A (ITM2A), a member of the Type II Integral Membrane protein (ITM2) family, has been reported
Yu-Wen Cao et al.
International journal of clinical and experimental pathology, 7(7), 4286-4294 (2014-08-15)
Accumulated evidence has revealed the presence of Notch receptor polymorphisms in non-tumorous diseases; however, few studies have investigated the association of Notch polymorphisms with breast cancer risk. A total of 100 invasive ductal carcinoma (IDC) and 50 ductal carcinoma in
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