SAB4700182
Monoclonal Anti-CD44-FITC antibody produced in mouse
clone MEM-85, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Anti-Pgp-1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.44
Recommended Products
biological source
mouse
Quality Level
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MEM-85, monoclonal
form
buffered aqueous solution
species reactivity
human
technique(s)
flow cytometry: suitable
isotype
IgG2b
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... CD44(960)
Related Categories
General description
The CD44 (cell-surface glycoprotein) gene with 20 exons is mapped to human chromosome 11p13. The gene codes for a transmembrane glycoprotein.
The antibody MEM-85 reacts with both cell surface-expressed and soluble form of CD44 antigen (Phagocyte glycoprotein 1), a 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes.
Immunogen
Leukocytes of a patient suffering from LGL Type Leukaemia.
Application
Monoclonal Anti-CD44-FITC antibody produced in mouse has been used as a reagent to distinguish HeLa (cancer) cells from chondrocyte (normal) cells.
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 1e6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.
Biochem/physiol Actions
CD44 (cell-surface glycoprotein) is involved in cell-cell and cell-extracellular matrix interactions. It plays an important role in lymphocyte homing and lymphocyte activation. It also acts as a metastasis suppressor gene for prostatic cancer. Overexpression of CD44 leads to esophageal squamous cell carcinoma (ESCC). CD44 inhibits macrophage multinucleation by interacting with its ligands, hyaluronic acid, chondroitin sulfates and osteopontin. 100kDa form of CD44, facilitates the binding of poliovirus to HeLa cells. In addition, it also implicated in the infection of mononuclear phagocytes by human immunodeficiency virus (HIV).
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Journal of Materials Chemistry, 22, 20918?20928-20918?20928 (2012)
Musashi-1 promotes a cancer stem cell lineage and chemoresistance in colorectal cancer cells.
Chiou GY
Scientific Reports, 7 (2017)
CD44 occupancy prevents macrophage multinucleation.
Sterling H
The Journal of Cell Biology, 143, 837-847 (1998)
Autophagy supports generation of cells with high CD44 expression via modulation of oxidative stress and Parkin-mediated mitochondrial clearance
Whelan KA
Oncogene, 36, 4843-4858 (2017)
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