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SAB5801052

Sigma-Aldrich

Goat Anti-Chicken IgY Alpaca Heavy chain only Antibody

recombinant Alpaca(VHH) Nano antibody

Synonym(s):

Recombinant Alpaca single variable domain secondary Nanobody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

alpaca

recombinant

expressed in HEK 293 cells (Human cell line)

conjugate

unconjugated

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

recombinant monoclonal

form

lyophilized solid

mol wt

100 kDa

species reactivity

chicken, Alpaca

technique(s)

ELISA: 1:10000-1:50000
western blot: 1:10000-1:50000

size

0.1 mL

color

colorless

isotype

VHH

shipped in

wet ice

storage temp.

2-8°C

General description

Goat Anti-Chicken IgY Alpaca Heavy chain only Antibody is designed to specifically detect chicken IgY. It is a monovalent, single domain, recombinant antibody fused to goat IgG Fc. Based on immunoelectrophoresis and/or ELISA, Goat Anti-Chicken IgY Alpaca Heavy chain only Antibody reacts with chicken IgY selectively but not with mouse, rabbit, human, cynomolgus, rat, goat IgG.

Hens egg yolk immunoglobulins IgY can be transferred from the serum of mother hen to the offspring egg yolk to acquire immunity. In the immunodiagnostic technologies, IgY is an excellent antibody for using in immunological assays involving mammalian sera, due to discriminative properties of IgY compared to mammalian IgG, as IgY does not react with the rheumatoid factor and human anti-mouse IgG antibodies do not activate the complement system and do not bind to Fc receptor. Also, they have poor cross reactivity to mammalian IgG due to immunological differences. IgY does not contain a hinge region but does contain an additional constant domain. The whole IgY molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgYcontains two heavy and light chains. The common IgY is monomeric with a molecular weight of approximately 170 kDa.

VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Specificity

Fc region of chicken IgY
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc

Immunogen

Recombinant Alpaca single variable domain secondary nano antibody

Packaging

100 μg in Glass bottle

Physical form

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Reconstitution

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Storage and Stability

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

Other Notes

No cross-reactivity with mouse, rabbit, human, cynomolgus, rat, goat IgG

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317,H412

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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