SHC003
MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA
Green fluorescent protein marker to monitor transduction efficiency
Synonym(s):
MISSION® Control Vectors
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About This Item
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Quality Level
product line
MISSION®
concentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in
dry ice
storage temp.
−20°C
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General description
The MISSION® TurboGFP™ Control Vector is an 8347 base pair lentivirus plasmid vector that contains a gene encoding TurboGFP™, under the control of the CMV promoter. The TurboGFP™ Control Vector is useful as a positive transfection/transduction control in experiments using the MISSION® shRNA library clones. TurboGFP™ is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA has been used to construct GATA2 overexpression vector (oeG2), by removing the GFP cassette of this vector at the 5′ NheI and 3′ PstI cloning sites and replacing it with the full-length GATA2 transcript sequence.
To see more application data, protocols, vector maps visit sigma.com/shrna.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboBeads is a trademark of TurboBeads LLC
TurboGFP is a trademark of Evrogen Co.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
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