SRP0115
Sirtuin 1 human
recombinant, expressed in E. coli, ≥60% (SDS-PAGE)
Synonym(s):
SIR2, SIRT1, Sir2A, sir2-like 1
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
biological source
human
recombinant
expressed in E. coli
assay
≥60% (SDS-PAGE)
form
aqueous solution
mol wt
87.2 kDa
packaging
pkg of 100 μg
storage condition
avoid repeated freeze/thaw cycles
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... SIRT1(23411)
General description
Human Sirtuin 1, GenBank Accession No. NM_012238), amino acids 193-741 with N-terminal ST tag, MW = 87.2 kDa, expressed in E. coli expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Unit Definition
One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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