SRP2024
PC4, serine mutations human
recombinant, expressed in E. coli, ≥80% (SDS-PAGE)
Synonym(s):
MGC102747, P15, PC4, p14
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.26
biological source
human
recombinant
expressed in E. coli
assay
≥80% (SDS-PAGE)
form
frozen liquid
mol wt
~14.4 kDa
packaging
pkg of 10 μg
concentration
1000 μg/mL
color
clear colorless
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... SUB1(10923)
Biochem/physiol Actions
Human PC4 is a non-TAF transcription coactivator that mediates activator-dependent transcription by RNA polymerase II in vitro through most tested activators. The function of PC4 is through interactions with transcriptional activators and the basal transcription machinery. It is negatively regulated by casein kinase II phosphorylation both in vitro and in vivo. PC4 strongly binds single stranded DNA and the region essential for the single stranded DNA binding activity was mapped around residue 77. A single amino acid change at position 77 (F to P) abolishes both ds- and ss-DNA binding activity.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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H Ge et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(26), 12691-12695 (1994-12-20)
Human positive cofactor 4 (PC4) mediates activator-dependent transcription by RNA polymerase II, apparently through interactions with transcriptional activators and the basal transcription machinery. We report here that PC4 function is modulated by in vivo phosphorylation. Protein-protein interaction studies and in
M Kretzschmar et al.
Cell, 78(3), 525-534 (1994-08-12)
Our investigations of mammalian class II gene transcription resulted in identification, purification, and cloning of the corresponding cDNA of a cellular factor (p15) that mediates the effects of several distinct activators on transcription in vitro. Functional deletion analyses revealed a
H Ge et al.
Cell, 78(3), 513-523 (1994-08-12)
Activator-dependent transcription in mammalian cells requires upstream stimulatory activity (USA)-derived cofactors in addition to those present in TFIID. A novel positive cofactor (PC4) purified from the human USA fraction effected a marked enhancement (up to 85-fold) of GAL4-AH-dependent transcription in
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