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SRP5280

Sigma-Aldrich

PI3K (p110-α/p85-α), active, His tagged from mouse and human

PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

p110-alpha, p85-alpha

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About This Item

CAS Number:
115926-52-8
UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human
mouse

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO®

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

64-97 nmol/min·mg

mol wt

~111 kDa (p110α)
 ~86 kDa (p85α)

NCBI accession no.

NM_008839

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

mouse ... Pik3ca(18706)

General description

The PI3K comprises of a 110 kDa catalytic subunit and a 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. The p110 alpha catalytic subunit (PIK3CA) is frequently mutated or amplified in a variety of cancers including ovarian and colon. PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of p110 alpha is essential for vascular development and inactivation of p110 alpha leads to severe defects in angiogenic sprouting and vascular remodeling.

Physical form

Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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