SRP6489
Carbonic Anhydrase 4/CA4 human
recombinant, expressed in HEK 293 cells, ≥92% (SDS-PAGE)
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.77
Recommended Products
biological source
human
recombinant
expressed in HEK 293 cells
tag
6-His tagged (C-terminus)
assay
≥92% (SDS-PAGE)
form
lyophilized
mol wt
calculated mol wt 31 kDa
observed mol wt 33 kDa by SDS-PAGE (reducing) (Ala 19 is the predicted N-terminal .)
packaging
pkg of 10 μg
impurities
<1 EU/μg endotoxin (LAL test)
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... CA4(762)
General description
Carbonic anhydrase 4 (CA4) is a 35kDa protein, encoded by the gene mapped to human chromosome 17q23.1-23.2. It is expressed on extracellular surfaces of certain endothelial and epithelial cells. CA4 is one among the 12 isozymes of carbonic anhydrase.
Carbonic anhydrase 4 (CA4) is also known as Carbonate dehydratase IV, Carbonic anhydrase IV, which belongs to the α-carbonic anhydrase family. CA4 may stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. The enzyme regulation is activated by histamine, L-adrenaline, D-phenylalanine, L- and D-histidine. CA4 can interact with SLC4A4.
Biochem/physiol Actions
Carbonic anhydrase 4 (CA4) plays a vital role in CO2 hydration activity. It also catalyzes the dehydration of HCO3. In addition, it also facilitates CO2 and HCO3 transport in various vital tissues like kidney and lung Polymorphism in the gene has been associated with the development of sporadic retinitis pigmentosa (RP).
Physical form
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Familial microduplication of 17q23.1?q23.2 involving TBX4 is associated with congenital clubfoot and reduced penetrance in females.
Peterson J F, et al.
American Journal of Medical Genetics. Part A, 164(2), 364-369 (2014)
Screening for the Carbonic Anhydrase IV Gene Mutations in Chinese Retinitis Pigmentosa Patients
Tian Y, et al.
Current Eye Research, 35(5), 440-444 (2010)
Membrane associated carbonic anhydrase IV (CA IV): a personal and historical perspective.
Waheed A, et al.
Sub-Cellular Biochemistry, 157-179 (2014)
Rupak Datta et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(9), 3437-3442 (2009-02-13)
Missense mutations in the carbonic anhydrase IV (CA IV) gene have been identified in patients with an autosomal dominant form of retinitis pigmentosa (RP17). We used two transient expression systems to investigate the molecular mechanism by which the newly identified
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