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SRP8001

Sigma-Aldrich

Calreticulin human

recombinant, expressed in E. coli, His tagged, >90% (SDS-PAGE)

Synonym(s):

CRP55, Calregulin, ERp60, Endoplasmic reticulum resident protein 60, grp60

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

tag

His tagged

assay

>90% (SDS-PAGE)

form

liquid

mol wt

~55 kDa by SDS-PAGE

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

calcium flux assay: suitable

impurities

<1 EU/μg endotoxin, tested

UniProt accession no.

P27797

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CALR(811)

General description

Calreticulin (CALR) is expressed in the endoplasmic reticulum. The gene encoding it is localized on human chromosome 19p13.13.

Application

Human calreticulin has been used in a study to determine the effects of uncoupling protein 4 (UCP4) on the expression of proteins in the store-operated Ca2+ entry pathway.

Biochem/physiol Actions

Calreticulin is involved in regulation of intracellular Ca2+ homoeostasis and ER Ca2+ capacity. It constitutes together with calnexin and ERp57 the ′calreticulin/calnexin cycle′ that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule. Recently, Calreticulin was shown to enhance the merger of macrophages and tumor cells, increasing phagocytosis.

Physical form

Solution in 55 mM TRIS-HCl, pH 8.2, containing 150 mM NaCl.

Other Notes

Human calreticulin (aa18-417) is fused at the C-terminus to a His-tag.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Calreticulin Is Involved in Invasion of Human Extravillous Trophoblasts Through Functional Regulation of Integrin ?1.
Yamamoto M
Endocrinology (2017)
CALR exon 9 mutations are somatically acquired events in familial cases of essential thrombocythemia or primary myelofibrosis
Elisa Rumi
Blood, 123 (2014)
A novel signalling screen demonstrates that CALR mutations activate essential MAPK signalling and facilitate megakaryocyte differentiation.
Kollmann K
Leukemia, 31(4) (2017)
Mitochondrial Uncoupling Protein-4 Regulates Calcium Homeostasis and Sensitivity to Store Depletion-induced Apoptosis in Neural Cells*
Sic. L. Chan
The Journal of Biological Chemistry (2006)
New microdeletion and microduplication syndromes: a comprehensive review
Julian Nevado
Genetics and Molecular Biology (2014)
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