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T0388

Sigma-Aldrich

Tartrazine

Dye content ≥85 %

Synonym(s):

Acid Yellow 23

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About This Item

Empirical Formula (Hill Notation):
C16H9N4Na3O9S2
CAS Number:
1934-21-0
Molecular Weight:
534.36
Colour Index Number:
19140
Beilstein/REAXYS Number:
69850
EC Number:
217-699-5
MDL number:
MFCD00148908
UNSPSC Code:
12352200
PubChem Substance ID:
24899885
NACRES:
NA.47

form

powder

Quality Level

200

composition

Dye content, ≥85%

color

orange

solubility

H2O: 10 mg/mL, clear, yellow to orange

density

2.12 at 20 °C

εmax

435-500 at 425-429 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[O-]C(=O)C1=NN(c2ccc(cc2)S([O-])(=O)=O)C(=O)C1N=Nc3ccc(cc3)S([O-])(=O)=O

InChI

1S/C16H12N4O9S2.3Na/c21-15-13(18-17-9-1-5-11(6-2-9)30(24,25)26)14(16(22)23)19-20(15)10-3-7-12(8-4-10)31(27,28)29;;;/h1-8,13H,(H,22,23)(H,24,25,26)(H,27,28,29);;;/q;3*+1/p-3

InChI key

UJMBCXLDXJUMFB-UHFFFAOYSA-K

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General description

Tartrazine (Cl 19140) also known as Acid yellow 23, is a hydrophilic polyanionic dye. It is a sulfonic dye, which can be synthesized by the reaction of sulfanilic acid with 3-carboxy-l-(4-sulfophenyl)-5-pyrazolone.

Application

Tartrazine may be used as a reference standard in the determination of tartrazine in food samples using ion-pair liquid chromatography coupled with photodiode-array and electrospray mass spectrometry detector.
The application of Tartrazine is seen in the phloxine-tartrazine procedure for cellular inclusions such as viral aggregates and eosinophil granules. It may be used as a substitute for orange G in a variant of Mallory′s trichrome, and in a methyl violet-tartrazine mixture to stain cryptosporidia in faecal smears and mucosal scrapings.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Simultaneous analysis of tartrazine and its intermediates by reversed phase liquid chromatography.
Wittmer PD, et al.
Analytical Chemistry, 47(8), 1422-1423 (1975)
Stella A Ordoudi et al.
Food chemistry, 267, 410-419 (2018-06-24)
The stepwise approach takes advantage of simple, versatile, low-cost screening tools that can be applied to several posts of the saffron trade chain to specifically detect adulteration with carminic acid (CA). This natural dye is of insect origin and should
Determination of sulphonated azo dyes in food by ion-pair liquid chromatography with photodiode array and electrospray mass spectrometry detection.
Fuh R-N and Chia J-K
Talanta, 56(4), 663-671 (2002)
Helen Waechter et al.
Analytical chemistry, 83(7), 2719-2725 (2011-03-02)
We demonstrate a method for measuring optical loss simultaneously at multiple wavelengths with cavity ring-down spectroscopy (CRD). Phase-shift CRD spectroscopy is used to obtain the absorption of a sample from the phase lag of intensity modulated light that is entering
Helen Waechter et al.
Analytical chemistry, 81(21), 9048-9054 (2009-10-10)
Analytical UV absorption detection for microfluidic devices, capillary electrophoresis, and even high-performance liquid chromatography is hampered by the small detection volumes, short absorption paths, and the need to sample at a high rate with a stable background and low noise.
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Protocols

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Separation of Tartrazine; Amaranth; Indigo carmine; New Coccine; Sunset Yellow FCF; Allura Red AC; Fast Green FCF; Erioglaucine disodium salt; Erythrosin B sodium salt; Phloxine B; Rose bengal

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