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T0951

Sigma-Aldrich

Anti-Tet Repressor (TETR) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Tetracycline Repressor Protein

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About This Item

MDL number:
MFCD08705524
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~22 kDa by calculation

species reactivity

human

technique(s)

indirect ELISA: suitable
western blot: 1:1,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

General description

Tet Repressor (TETR) is homodimer comprising a C-terminal helix-turn-helix motif (HTH) and N-terminal DNA-binding domain,. It also has a C-terminal tetracycline-binding and dimerization (TBD) domain.

Immunogen

Anti-Tet Repressor (TETR) reacts with amino acid residues 31-49 (AQKLGVEQPTLYWHVKNKR) of 22 kDa Tet repressor protein

Application

Anti-Tet Repressor (TETR) antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

The Tet system is employed in conditional gene expression studies in eukaryotic cells. TetR ( can be used for such applications in some organisms without further modifications. In mammals and in a large variety of other organisms, however, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator. TetR interaction with antibiotic tetracyclin decreases its binding affinity to DNA.

Physical form

Solution of 1 mg/mL in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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The TetR family of transcriptional repressors
Ramos JL, et al.
Microbiology and Molecular Biology Reviews, 69(2), 326-356 (2005)
E Pook et al.
European journal of biochemistry, 258(3), 915-922 (1999-02-17)
We isolated five monoclonal antibodies (mAbs) made against tetracycline repressor (TetR), one against the TetR tetracycline complex (Tc) and two against the TetR-tet operator (tetO) complex. The epitopes of the anti-TetR mAbs are localized in the alpha-helix-turn-alpha-helix motif (HTH), at
FOXO1 is an essential regulator of pluripotency in human embryonic stem cells
Zhang X, et al.
Nature Cell Biology, 13(9), 1092-1092 (2011)
W Hillen et al.
Annual review of microbiology, 48, 345-369 (1994-01-01)
Tetracycline-resistance determinants encoding active efflux of the drug are widely distributed in gram-negative bacteria and unique with respect to genetic organization and regulation of expression. Each determinant consists of two genes called tetA and tetR, which are oriented with divergent
The application of Tet repressor in prokaryotic gene regulation and expression
Bertram R and Hillen W
Microbial Biotechnology, 1(1), 2-16 (2008)
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