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T1819

Sigma-Aldrich

Trizma® hydrochloride solution

pH 7.0, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

grade

BioPerformance Certified
for molecular biology

Quality Level

200

form

liquid

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

7.0

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

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General description

Tris(hydroxymethyl)aminomethane (Tris) is a standard buffer in biological sciences and has a pH buffering range between 7.0 - 9.0. It is applicable in electrophoretic studies and capillary electrochromatography.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma® hydrochloride solution has been used:
  • as a buffer in reverse transcription and DNase I treatment reactions for RNA isolated from Toxoplasma gondii
  • in the preparation of tris- ethylenediaminetetraacetic acid (EDTA) (TE) buffer for bacterial pellet resuspension for plasmid isolation studies
  • for polylactic acid nanoparticle resuspension, storage, and pH-based stability studies

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A review of acellular immersion tests on bioactive glasses-influence of medium on ion release and apatite formation
Nommeots-Nomm A, et al.
International Journal of Applied Glass Science, 11(3) (11)
Kaichun Zhu et al.
Nature protocols, 1(6), 3088-3093 (2007-04-05)
This protocol describes a streamlined method of plasmid DNA extraction by continual thermal lysis, a modification of the basic boiling lysis technique, to simplify the processing of large volumes of Escherichia coli cultures. Fermented bacteria are harvested using a hollow

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