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T7451

Sigma-Aldrich

Anti-Acetylated Tubulin antibody, Mouse monoclonal

clone 6-11B-1, purified from hybridoma cell culture

Synonym(s):

Acetylated Tubulin Antibody, Acetylated Tubulin Antibody - Monoclonal Anti-Acetylated Tubulin antibody produced in mouse, Anti Acetyl Tubulin antibody, Anti Acetyl-alpha tubulin

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About This Item

MDL number:
MFCD00164512
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6-11B-1, monoclonal

form

buffered aqueous solution

species reactivity

bovine, frog, invertebrates, human, hamster, mouse, protista, pig, monkey, chicken, rat, plant

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

dot blot: suitable
electron microscopy: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: 0.03-0.06 μg/mL using total rat brain extract

isotype

IgG2b

UniProt accession no.

P68366

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

acetylation (Lys40)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Related Categories

General description

α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α -tubulin family containing 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.
Monoclonal anti-acetylated tubulin antibody (mouse IgG2b isotype) is derived from the hybridoma 6-11B-1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin). Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 50,000 and share considerable homology.

Specificity

The antibody has been used to detect acetylated α-tubulins from many organisms including protista, plants, invertebrates, and vertebrates. The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys40 when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corre­sponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila, and yeast. Occasionally, the epitope recognized by the antibody may be absent or masked, as it is in the rat kangaroo epithelial-like cell line PtK2.

Immunogen

acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin).

Application

Monoclonal Anti-Acetylated Tubulin antibody produced in mouse has been used in:
  • quantitative dot blot
  • immunofluorescence
  • Western blot
  • immunocytochemistry
  • enzyme-linked immunosorbent assay (ELISA)
  • solid-phase radioimmunoassay (RIA)
  • electron microscopy
Anti-acetylated tubulin antibody has also been used in staining X-gal–stained paraffin sections to perform immunohistochemistry.

Biochem/physiol Actions

Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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3D spheroid model of mIMCD3 cells for studying ciliopathies and renal epithelial disorders
Giles RH, et al.
Nature Protocols, 9(12), 2725-2725 (2014)
M LeDizet et al.
Proceedings of the National Academy of Sciences of the United States of America, 84(16), 5720-5724 (1987-08-01)
An acetylation site of Chlamydomonas axonemal alpha-tubulins was identified near, or within, the binding site of 6-11B-1, a monoclonal antibody specific for posttranslationally acetylated alpha-tubulins. In a first approach, axonemal proteins were hydrolyzed by formic acid, cyanogen bromide, or chymotrypsin
All tubulins are not alike: heterodimer dissociation differs among different biological sources
Montecinos-Franjola F, et al.
The Journal of biological chemistry, 9(12), jbc-RA119 (2019)
Basal cells as stem cells of the mouse trachea and human airway epithelium.
Rock JR
Proceedings of the National Academy of Sciences of the USA, 106(31), 12771-12775 (2009)
G Piperno et al.
The Journal of cell biology, 104(2), 289-302 (1987-02-01)
The subcellular distribution of microtubules containing acetylated alpha-tubulin in mammalian cells in culture was analyzed with 6-11B-1, a monoclonal antibody specific for acetylated alpha-tubulin. Cultures of 3T3, HeLa, and PtK2 cells were grown on coverslips and observed by immunofluorescence microscopy
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