Skip to Content
MilliporeSigma
All Photos(1)

Documents

U3383

Sigma-Aldrich

Urease Buffer Reagent

Synonym(s):

Urea colorimetric determination assay reagent

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

description

When reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative.

Quality Level

200

form

powder

mol wt

545 kDa

storage temp.

2-8°C

General description

Urease is a 545 kDa protein critical for the hydrolysis of urea to carbon dioxide and ammonia.
Urease is a dinickel enzyme that hydrolyses urea into ammonia and carbon dioxide. It is secreted by several bacterial species, invertebrates, fungi, and plants. Detection of urease activity is a potential diagnostic tool for Helicobacter pylori infections and several oral bacterial infections. two Ni2+ ions bridged by a hydroxyl group and a carbamylated lysine.

Application

Urease Buffer Reagent has been used:
  • to measure plasma urea N (PUN) by the hypochlorite-phenol nitroprusside method
  • in cell viability assay to measure the synthesis of urea in human fetal stem/progenitor cells
  • to study the metabolic capacity of rat hepatocytes

Used in conjunction with Phenol Nitroprusside solution (Product Code P 6994) and Alkaline Hydpochlorite solution (Product Code A 1727) for the colorimetric determination of urea.

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Slide 1 of 8

1 of 8

Urease from Canavalia ensiformis (Jack bean) Type III, glycerol solution, 500-800 units/mL

Sigma-Aldrich

U1875

Urease from Canavalia ensiformis (Jack bean)

Urease from Canavalia ensiformis (Jack bean) Type C-3, powder, ≥600,000 units/g solid

Sigma-Aldrich

U0251

Urease from Canavalia ensiformis (Jack bean)

Jack beans fine powder

Sigma-Aldrich

J0125

Jack beans

Sigma-Aldrich

Sigma-Aldrich

A1727

Alkaline Hypochlorite solution

Sodium nitroprusside British Pharmacopoeia (BP) Reference Standard

BP453

Sodium nitroprusside

Sigma-Aldrich

Sigma-Aldrich

49291

Glucosidase from Aspergillus niger

Phosphatase, Acid from wheat germ ≥0.4 unit/mg solid

Sigma-Aldrich

P3627

Phosphatase, Acid from wheat germ

Ammonia Assay Kit sufficient for 100 assays

Sigma-Aldrich

AA0100

Ammonia Assay Kit

Cristian Follmer
Phytochemistry, 69(1), 18-28 (2007-08-21)
The broad distribution of ureases in leguminous seeds, as well as the accumulation pattern of the protein during seed maturation, are suggestive of an important physiological role for this enzyme. Since the isolation and characterization of jack bean urease by
Kang Cheng et al.
Hepatology (Baltimore, Md.), 50(4), 1194-1203 (2009-07-29)
Tracking stem/progenitor cells through noninvasive imaging is a helpful means of assessing the targeting of transplanted cells to specific organs. We performed in vitro and in vivo studies wherein adult human hepatocytes and human fetal liver stem/progenitor cells were labeled
Yuta Enami et al.
Hepatology (Baltimore, Md.), 55(4), 1182-1192 (2011-10-14)
Organs from non-heart-beating donors are attractive for use in cell therapy. Understanding the nature of molecular perturbations following reperfusion/reoxygenation will be highly significant for non-heart-beating donor cells. We studied non-heart-beating donor rats for global gene expression with Affymetrix microarrays, hepatic
L A Winkelman et al.
Journal of dairy science, 96(12), 7565-7577 (2013-10-15)
This study investigated the effect of 2 different types of long-acting insulin on milk production, milk composition, and metabolism in lactating dairy cows. Multiparous cows (n=30) averaging 88 d in milk were assigned to one of 3 treatments in a
Rukhsana Aslam et al.
Histology and histopathology, 35(12), 1483-1492 (2020-10-31)
To determine the role of the transplantation of bone marrow-derived mesenchymal stem cells (MSCs) in podocyte renewal, we studied BALB/C mice with or without adriamycin-induced acute kidney injury. MSCs were transplanted ectopically under the capsule of the left kidney or

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service