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WH0003845M1

Sigma-Aldrich

Monoclonal Anti-KRAS antibody produced in mouse

clone 3B10-2F2, purified immunoglobulin, buffered aqueous solution

Synonym(s):

KRAS Antibody - Monoclonal Anti-KRAS antibody produced in mouse, Kras Antibody, Anti-CKRAS, Anti-KIRAS, Anti-KRAS1, Anti-KRAS2, Anti-KRAS2A, Anti-KRAS2B, Anti-KRAS4A, Anti-KRAS4B, Anti-RASK2, Anti-v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

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About This Item

MDL number:
MFCD00239650
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3B10-2F2, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

ELISA: suitable
capture ELISA: suitable
immunofluorescence: suitable
western blot: 1-5 μg/mL

isotype

IgG1κ

GenBank accession no.

BC013572

UniProt accession no.

P01116

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... KRAS(3845)

General description

Kirsten rat sarcoma viral oncogene homologue (KRAS) is an oncogene that is mapped to human chromosome 12p12.1. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. The gene codes for a member of the small GTPase superfamily.

Immunogen

KRAS (AAH13572, 1 a.a. ~ 188 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence
MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM

Application

Monoclonal Anti-KRAS antibody produced in mouse has been used in immunoprecipitation, immunofluorescence, western blotting, indirect enzyme linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Kirsten rat sarcoma viral oncogene homologue (KRAS) is a key protein of the Ras signaling pathways. It facilitates the invasion and metastasis of tumors. Gain-of-function mutations in the gene leads to the development of variety of tumors, including pancreatic, biliary tract and colon tumors. This mutation is rarely observed in gastric cancer.

Physical form

Solution in phosphate buffered saline, pH 7.4

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

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Atorvastatin overcomes gefitinib resistance in KRAS mutant human non-small cell lung carcinoma cells
Chen J, et al
Cell Death & Disease, 4(9), e814-e814 (2013)
MiR-384 inhibits human colorectal cancer metastasis by targeting KRAS and CDC42
Wang YX, et al.
Oncotarget, 7, 84826-84826 (2016)
Mahmoud Toulany et al.
Cancer biology & therapy, 15(3), 317-328 (2013-12-20)
K-RAS mutated (K-RASmut) non-small cell lung cancer (NSCLC) cells are resistant to EGFR targeting strategies. We investigated the impact of K-RAS activity irrespective of mutational status in the EGFR-independent increase in clonogenic cell survival. An analysis of the K-RAS activity
Andrew M Waters et al.
PloS one, 11(9), e0163272-e0163272 (2016-09-30)
Synonymous mutations in the KRAS gene are clustered at G12, G13, and G60 in human cancers. We constructed 9 stable NIH3T3 cell lines expressing KRAS, each with one of these synonymous mutations. Compared to the negative control cell line expressing
Loss of heterozygosity of chromosome 12p does not correlate with KRAS mutation in non-small cell lung cancer
Uchiyama M, et al.
International Journal of Cancer. Journal International Du Cancer, 107, 962-969 (2003)
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