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X0629

Sigma-Aldrich

Anti-XRCC1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-X-ray repair complementing defective repair in Chinese hamster cells 1

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About This Item

MDL number:
MFCD01865396
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 85 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using MCF7 cells fixed with paraformaldehyde-Triton
western blot: 0.5-1 μg/mL using A431 cell lysates

UniProt accession no.

P18887

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... XRCC1(7515)
mouse ... Xrcc1(22594)
rat ... Xrcc1(84495)

Related Categories

General description

X-ray repair cross-complementing group 1 (XRCC1) is a testis enriched protein. It is considered as a DNA repair gene.

Immunogen

synthetic peptide corresponding to amino acids 615-633 of human XRCC1, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is identical in mouse and rat.

Application

Anti-XRCC1 antibody produced in rabbit has been used in:
  • immunoblotting
  • immunofluorescence
  • indirect immunofluorescence and telomere FISH (TEL-FISH)
  • protein detection
  • western analysis

Biochem/physiol Actions

X-ray repair cross-complementing group 1 (XRCC1) is a molecular scaffold protein that coordinates the assembly of repair complexes at damaged sites. XRCC has been shown to physically interact with several enzymes known to be involved in the repair of SSBs, including DNA ligase IIIa, DNA polymerase b, APE1, polynucleotide kinase (PNK), poly (ADP-ribose), polymerases 1 and 2 (PARP-1 and 2) and others. It participates in the DNA base excision repair (BER) pathway.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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XRCC1 deficiency correlates with increased DNA damage and male infertility
Singh V, et al.
Mutation Research. Genetic Toxicology and Environmental Mutagenesis, 839, 1-8 (2019)
XRCC1 deficiency correlates with increased DNA damage and male infertility
Singh V, et al.
Mutation Research. Genetic Toxicology and Environmental Mutagenesis, 839 (2019)
Characterization of oxidative guanine damage and repair in mammalian telomeres
Wang Z, et al.
PLoS Genetics, 6(5), e1000951-e1000951 (2010)
XRCC1 polymorphism and overall survival in ovarian cancer patients treated with platinum-based chemotherapy: A systematic review and MOOSE-compliant meta-analysis
Zhang Z, et al.
Medicine, 97(45) (2018)
X-ray repair cross complementing protein 1 in base excision repair
Hanssen-Bauer A, et al.
International Journal of Molecular Sciences, 13(12), 17210-17229 (2012)
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