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Y1003

Sigma-Aldrich

2x YT medium

Liquid microbial growth medium

Synonym(s):

2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium

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About This Item

UNSPSC Code:
41106225
NACRES:
NA.85

grade

for molecular biology

Quality Level

300

sterility

sterile; 0.2 μm filtered

form

liquid

technique(s)

microbiological culture: suitable

pH

6.5-7.5

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

2xYT ( 2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

2x YT medium has been used for non-selective cultivation of M13 bacteriophage, E. coli strains for cloning , DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

2xYT liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Components

16 g/L Tryptone
10 g/L Yeast Extract
5.0 g/L NaCl

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Certificates of Analysis (COA)

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