Anti-p16 Antibody, clone D25 ZooMAb^® Mouse Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

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Clone D25 is a ZooMAb^® mouse recombinant monoclonal antibody that specifically detects Cyclin-dependent kinase inhibitor 2A (p16).

Recombinant human p16 fusion protein.

Quality Control Testing

Evaluated by Western Blotting with human recombinant p16 (CDKN2A)..

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected human recombinant p16 (CDKN2A).

Tested applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p16 in HeLa cells.
Flow Cytometry Analysis: 1 μg from a representative lot detected p16 in Hela cells.

Affinity Binding Assay: A representative lot of this antibody bound recombinant human p16 (CDKN2A) with a KD of 2.5 x 10-6 in an affinity binding assay.

Flow Cytometry Analysis: 1 μg from a representative lot detected p16 (CDKN2A) in one million HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-p16, clone D25 ZooMAb^®, Cat. No. ZMS1072, is a recombinant Mouse monoclonal antibody that detects p16 (CDKN2A) and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, and Western Blotting.

Cyclin-dependent kinase inhibitor 2A (UniProt: P42771; also known as Cyclin-dependent kinase 4 inhibitor A, CDK4I, Multiple tumor suppressor 1, MTS-1, p16-INK4a, p16-INK4, p16INK4A) is encoded by the CDKN2A (also known as CDKN2, MTS1) gene (Gene ID: 1029) in human. p16 serves as a negative regulator of cell proliferation by strongly interacting with CDK4 and CDK6 during the G1-to S-phase transition and blocking the phosphorylation of retinoblastoma (Rb) protein. Hence, it is considered as a tumor suppressor protein. Hypo-phosphorylated Rb protein forms complexes with E2F family of transcription factors, which sequesters E2Fs and prevent their access to the promoters of proliferation-associated genes. During cell cycle, in the late G1 phase CDK4 and CDK6 hyper-phosphorylate Rb protein that commits cells to enter into S phase. Binding of p16 maintains Rb in a hypo-phosphorylated state. CDKN2A gene is reported to be one of the most frequently mutated genes and is reported to be inactivated in about 50% of all human cancers. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Li, J., et al. (2011). Biochemistry. 50(25); 5566-5582; Sherr, CJ., and Roberts, JM (2004). Genes Dev. 18(22); 2699-2711; Ortega, S., et al. (2002). Biochim. Biophys. Acta. 1602(1); 73-87).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.