Anti-RUNX2 Antibody, clone 1D6 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1D6 is a Rabbit recombinant monoclonal antibody that specifically detects Runt-related transcription factor 2 (RUNX2). It targets an epitope within 23 amino acids from the internal region

KLH-conjugated linear peptide corresponding to 23 amino acids from the internal region of human Runt-related transcription factor 2 (RUNX2).

Quality Control Testing

Evaluated by Western Blotting in C2C12 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected RUNX2 in C2C12 cell lysate.

Tested applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected RUNX2 in SAOS-2 cella.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected RUNX2 in human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound RUNX2 with a KD of 1.7 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Runt-related transcription factor 2 (UniProt: Q13950; also known as Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, CBF-alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, OSF-2, Polyomavirus enhancer-binding protein 2 alpha A subunit, PEA2-alpha A, PEBP2-alpha A, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, RUNX2) is encoded by the RUNX2 (also known as AML3, CBFA1, OSF2, PEBP2A) gene (Gene ID: 860) in human. RUNX2 is a master transcription factor that is specifically expressed in membranous and endochondral bones and is involved in osteoblastic differentiation and skeletal morphogenesis. Its expression in the bone and osteogenic front of a suture is crucial for cranial suture closure and membranous bone morphogenesis. It is a heterodimer of a and b subunits. The a subunit binds DNA as a monomer through its RUNT domain (aa 101-229). Its activity is shown to be precisely regulated by phosphorylation at multiple sites. Protein kinase C activated by FGF2 phosphorylates RUNX2 at serine 247 and ERK phosphorylates serine 301 in the PST domain that is reported to be critical for the enhancement of subsequent acetylation and the suppression of ubiquitination of the RUNX2 protein. Phosphorylation of serine 451 by CDK1 is reported to promotes endothelial cell proliferation. Mutations in RUNX2 gene have been linked to craniofacial malformations. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kim, WJ., et al. (2020). Exp. Mol. Med. 52(8); 1178-1184; Kawane, T., et al (2018). Sci. Rep. 8; Article 13551).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.