Anti-Hexokinase-2 Antibody, clone 1F3 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1F3 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Hexokinase -2. It targets an epitope within 22 amino acids from the N-terminal half.

KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal half of human Hexokinase-2.

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Hexokinase-2 in HeLa cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution of a representative lot detected Hexokinase-2 in HCT116 and RAW264.7 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of a representative lot detected Hexokinase-2 in human skeletal muscle tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Hexokinase-2 peptide with a KD of 2.2 x 10-6 in an affinity binding assay.

Flow Cytometry Analysis: 0.1 µg of a representative lot detected Hexokinase-2 in one million HeLa cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Hexokinase-2 (UniProt: P52789; also known as EC:2.7.1.1, Hexokinase type II, HK II, Hexokinase-B, Muscle form hexokinase) is encoded by the HK2 gene (Gene ID: 3099) in human. Hexokinase-2 is a monomeric enzyme that is predominantly expressed in insulin-responsive tissues. It catalyzes the phosphorylation of glucose to produce glucose-6-phosphate, the initial step in glycolysis. It plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis. It is synthesized with a mitochondrial binding peptide (aa 1-16) and its N-and C-terminal halves contain a hexokinase domain, both of which display catalytic activity. Hexokinase-2 activity is elevated in tumor cells, which is crucial for the Warburg effect and for supporting the highly glycolytic nature of cancer cells.  The elevated amount of hexokinase-2 binds both the ATP and the incoming glucose to produce large amounts of glucose-6-phosphate that supports cell proliferation. In contrast to normal brain and low-grade glioma, which express hexokinase-1, glioblastoma multiforme has elevated levels of hexokinase-2 that correlates with worse overall survival rates. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wolf, A., et al. (2011). J. Exp. Med. 208(2); 313-326; Chiara, F., et al. (2008). PLoS One. 3(3); e1852; Mathupala,SP., et al. (2006). Oncogene. 25(34); 4777-4786).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.