Anti-Cardiac Troponin T Antibody, clone 1G6 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1G6 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Cardiac Troponin T. It targets an epitope within 19 amino acids from the internal region.

KLH-conjugated linear peptide corresponding to 19 amino acids from the internal region of human cardiac muscle Troponin T.

Quality Control Testing

Evaluated by Western Blotting in Human skeletal muscle tissue lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Cardiac Troponin T in Human skeletal muscle tissue lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Cardiac Troponin T in Human heart tissue sections.

Affinity Binding Assay:: A representative lot of this antibody bound Cardiac Troponin T peptide with a KD of 6.8 x 10-8 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution of from a representative lot detected Cardiac Troponin T in L6 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Troponin T, cardiac muscle (UniProt: P45379; also known as TnTc, Cardiac muscle troponin T, cTnT) is encoded by the TNNT2 gene (Gene ID: 7139) in human. Troponins are structural proteins in skeletal and cardiac muscle thin filaments. The troponin complex consists of three subunits (I, T, and C) that along with calcium ions plays an important role in the regulation of muscle contraction. Each of these troponins have a specific role in the muscle contraction. Troponin T attaches the troponin complex to the actin filament, troponin C acts as the calcium binding site, and troponin I inhibits interaction with myosin heads in the absence of sufficient calcium ions. Elevated levels of troponin T have been linked to a broad spectrum of cardiomyopathies. Its detection is considered as a more specific test for myocardial damage than creatine kinase but may not always indicate ischemic mechanism of injury. Troponin T is reported to split into two sub-fragments, troponin T1 (TnT1) and troponin T2

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.