Anti-B7-H3/CD276 Antibody, clone 1D4 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1D4 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects B7-H3/CD276. It targets an epitope within 21 amino acids from the extracellular domain within the N-terminal half.

KLH-conjugated linear peptide corresponding to 21 amino acids from the extracellular domain within the N-terminal half of human B7-H3/CD276.

Quality Control Testing

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected B7-H3/CD276 in HEK293 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected B7-H3/CD276 in PC3 and THP-1 cell lysates.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected B7-H3/CD276 in one million THP-1 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected B7-H3/CD276 in human tonsil tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected B7-H3/CD276 in THP-1 cells.

Affinity Binding Assay: A representative lot of this antibody bound B7-H3/CD276 with a KD of 4.9 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

CD276 antigen (UniProt: Q5ZPR3; also known as 4Ig-B7-H3, B7 homolog 3, B7-H3, Costimulatory molecule, CD276) is encoded by the CD276 (also known as B7H3, PSEC0249, UNQ309/PRO352) gene (Gene ID: 80381) in human. B7-H3/CD276 is a single-pass type I membrane protein of the B7 immunoregulatory family. It is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 29-466), a short transmembrane domain (aa 467-487), and a cytoplasmic domain (aa 488-534). It participates in the regulation of T-cell-mediated immune response. It is a ubiquitous protein but is undetectable in peripheral blood lymphocytes or granulocytes and is only weakly expressed in resting monocytes. It is also expressed in dendritic cells derived from monocytes and in epithelial cells of sinonasal tissue, extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta. It plays a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Higher expression of B7-H3/CD276 is observed in a variety of cancers, including breast, pancreatic, and ovarian cancer and its expression has been correlated with poor survival. It is suggested to play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis. Its levels are also reported to be up-regulated in cells that mediate rejection of human transplants. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Fauci, JM., et al. (2014). Gynecologic Oncology 132(1); 203-210; Chen, Y-W., et al. (2008). Curr. Can. Drug Targets. 8(5); 404-413).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.