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Astec® CHIROBIOTIC® V2 Chiral (5 μm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Synonym(s):

CHIROBIOTIC V2 Chiral Chromatography Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Astec® CHIROBIOTIC® V2 Chiral HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Quality Level

agency

suitable for USP L88

description

HPLC column

product line

Astec®

packaging

pkg of 1 ea

manufacturer/tradename

Astec®

parameter

0-45 °C temperature
241 bar pressure (3500 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

matrix

High-purity silica gel particle platform
fully porous particle

matrix active group

vancomycin phase

particle size

5 μm

pore size

200 Å

operating pH range

3.5-7.0

separation technique

chiral

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General description

Neutral molecules, amides, acids, esters and amines show considerable enantioselectivity on these vancomycin-based CSPs. A wide variety of secondary and tertiary amines have been separated on the CHIROBIOTIC® V in the polar ionic mode. The CHIROBIOTIC® V has demonstrated many of the separation characteristics of protein-based stationary phases, but with exceptional stability and much higher sample capacity. Some chiral analytes have been resolved that have not been reported separated on any other chiral stationary phase. CHIROBIOTIC® V and V2 differ in their bonding chemistry the pore size of the support particle, giving them different selectivity and preparative capacity.

  • Bonded phase: Vancomycin
  • Operating pH range: 3.5 - 7.0
  • Particle diameter: 5, 10 or 16 μm
  • Pore size: 100 Å (CHIROBIOTIC® V) or 200 Å (CHIROBIOTIC® V2)

CHIROBIOTIC FAQs
CHIROBIOTIC Reference Bibliography
Chiral Product Literature

Application


  • Development and validation of an UFLC-MS/MS method for enantioselectivity determination of d,l-thero-methylphenidate, d,l-thero-ethylphenidate and d,l-thero-ritalinic acid in rat plasma and its application to pharmacokinetic study.: This research showcases the Astec® CHIROBIOTIC® V2 Chiral HPLC Column′s capacity for precise enantioselective analysis in pharmacokinetic studies, specifically for psychoactive substances. It highlights the column′s effectiveness in separating and quantifying chiral drugs and their metabolites in plasma, providing valuable insights for drug development and therapeutic monitoring (Zhang et al., 2016).

  • New high-performance liquid chromatography method for the determination of (R)-warfarin and (S)-warfarin using chiral separation on a glycopeptide-based stationary phase.: This study illustrates the use of the Astec® CHIROBIOTIC® V2 Chiral HPLC Column for the chiral separation of warfarin enantiomers. The methodology developed provides a robust tool for the analysis of anticoagulant drugs, aiding in the assessment of their pharmacokinetics and optimizing dosage for therapeutic efficacy (Malakova et al., 2009).

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Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chromatography Liquid Chiral Separations
Xiao, T.L., et al.
Encyclopedia of Separation Science, 1-15 (2000)
Enantioselective and sensitive determination of carvedilol in human plasma using chiral stationary-phase column and reverse-phase liquid chromatography with tandem mass spectrometry
Jiang, Juanjuan, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 960, 92-97 (2014)
Enantioselective determination of doxazosin in human plasma by liquid chromatography?tandem mass spectrometry using ovomucoid chiral stationary phase
Liu, Ke, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 878 (26), 2415-2420 (2010)
Quantitative determination of ondansetron in human plasma by enantioselective liquid chromatography-tandem mass spectrometry
Liu, Ke, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 864 (1-2), 129-136 (2008)
Validation of a chiral liquid chromatography?tandem mass spectrometry method for the determination of pantoprazole in dog plasma
Chen, Meixia, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 906, 85-90 (2012)

Articles

Shown here is the chiral separation of amphetamine enantiomers under MS-compatible conditions on Astec CHIROBIOTIC® V2 after extraction from urine using Supel™-Select SCX SPE 96-well plates. The highest grade mobile phase solvents and additives were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Protocols

Optimized sample prep and chiral chromatography methods for the LC/MS analysis of these drug enantiomers in urine

In this study, optimized methods are presented for sample preparation and chiral chromatography for the LC/MS analysis of amphetamine and methamphetamine enantiomers in urine.

LC/MS Analysis of Methamphetamine Enantiomers on Astec® CHIROBIOTIC® V2 in Urine after SPE using Supel™-Select SCX

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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