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Supelco

Supelclean™ LC-18 SPE Tube

bed wt. 100 mg, volume 1 mL, pk of 108

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About This Item

UNSPSC Code:
41115712

material

PE frit (20 μm porosity)
polypropylene hardware

Quality Level

100

product line

Supelclean™

feature

endcapped

composition

bed wt., 100 mg

packaging

pk of 108

extent of labeling

~11.5% C loading

technique(s)

solid phase extraction (SPE): suitable

surface area

475 m2/g

volume

1 mL

matrix

silica gel base material (irregularly shaped)

matrix active group

C18 (octadecyl) bonding, monomerically bonded

particle size

45 μm

pore size

0.8 cm3/g pore volume
60 Å pore size

application(s)

food and beverages

separation technique

reversed phase

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General description

Supelclean™ LC-18 SPE tube is used in solid phase extraction (SPE) method to extract non-polar to moderately polar compounds from aqueous samples.

Application

Supelclean™ LC-18 SPE Tube may be used for the solid-phase extraction of:
  • Artesunate, dihydroartemisinin, mefloquine, amodiaquine and desethylamodiaquine in human plasma samples prior to their determination by reversed phase high performance liquid chromatography (RP-HPLC) coupled to electrochemical (EC) detector or ultraviolet (UV) detector.
  • Ursodeoxycholic acid in blood serum samples prior to its analysis by HPLC-UV.
  • Calycosin-7-O-β-D-glucoside, ononin, astragaloside IV, astragaloside I and ferulic acid in rat plasma samples after oral administration of Danggui Buxue Tang extract, prior to their pharmacokinetic studies by LC coupled to mass spectrometry (MS).

Legal Information

Supelclean is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Validation of high performance liquid chromatography-electrochemical detection methods with simultaneous extraction procedure for the determination of artesunate, dihydroartemisinin, amodiaquine and desethylamodiaquine in human plasma for application in clinical pharmacological studies of artesunate-amodiaquine drug combination.
Lai CS, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 877(5-6), 558-562 (2009)
Simultaneous determination of calycosin-7-O-β-D-glucoside, ononin, astragaloside IV, astragaloside I and ferulic acid in rat plasma after oral administration of Danggui Buxue Tang extract for their pharmacokinetic studies by liquid chromatography-mass spectrometry.
Wen XD, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 865(1-2), 99-105 (2008)
An analytical method with a single extraction procedure and two separate high performance liquid chromatographic systems for the determination of artesunate, dihydroartemisinin and mefloquine in human plasma for application in clinical pharmacological studies of the drug combination.
Lai CS, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 857(2), 308-314 (2007)
Barbara Sivakova et al.
International journal of molecular sciences, 22(4) (2021-02-13)
The phosphorylation of proteins modulates various functions of proteins and plays an important role in the regulation of cell signaling. In recent years, label-free quantitative (LFQ) phosphoproteomics has become a powerful tool to analyze the phosphorylation of proteins within complex
M Nobilis et al.
Journal of pharmaceutical and biomedical analysis, 24(5-6), 937-946 (2001-03-15)
Ursodeoxycholic acid (3 alpha,7 beta-dihydroxy-5 beta-cholanoic acid, UDCA) is a therapeutically applicable bile acid widely used for the dissolution of cholesterol-rich gallstones and in the treatment of chronic liver diseases associated with cholestasis. UDCA is more hydrophilic and less toxic

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Solid Phase Extraction: Reversed-Phase Methodology

Reversed-phase interaction will retain most molecules with hydrophobic character; it is very useful for extracting analytes that are very diverse in structure within the same sample.

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