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Ascentis® Express OH5, 2 μm UHPLC Column

2 μm particle size, L × I.D. 5 cm × 2.1 mm

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

stainless steel column

Quality Level

100

agency

suitable for USP L86

product line

Ascentis®

feature

endcapped: no

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

≤60 °C temp. limit
1000 bar max. pressure (14500 psi)

technique(s)

LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

5 cm × 2.1 mm

surface area

120 m2/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform
superficially porous particle

matrix active group

hydroxy, penta- phase

particle size

2 μm

pore size

90 Å pore size

pH range

2-9

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)

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General description

The Ascentis® Express 2μm OH5 stationary phase is a highly polar ligand that possesses 5 hydroxyl groups tethered to the silica via novel proprietary linkage chemistry. This high performance material provides a UHPLC column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, zwitterionic, or neutral compounds.

Application

UHPLC/MS Analysis of Ethanol Metabolites in Urine on Ascentis® Express OH5

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificates of Analysis (COA)

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USYGV001311
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USYGV001307
USYGV001306

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Paweł Kubica et al.
Analytical and bioanalytical chemistry, 406(13), 3013-3018 (2014-03-26)
The influence of sucrose combustion products on smoking and nicotine addiction is still controversial because the presence of the sucrose may be treated as a source of aldehydes and organic acids. In e-liquids used as refills for electronic cigarettes, which

Articles

Ascentis® Express OH5 U/HPLC Columns

High-speed Ascentis® Express OH5 HPLC columns are designed for enhanced HILIC partitioning and limited ion exchange retention

Related Content

Small Molecule HPLC

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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