50994-U
BIOshell Glycan (2.7 μm) HPLC Columns
L × I.D. 15 cm × 2.1 mm, HPLC Column
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product name
BIOshell Glycan HPLC Columns, L × I.D. 15 cm × 2.1 mm, 2.7 μm particle size
material
stainless steel hardware (column)
Quality Level
agency
suitable for USP L86
description
shell thickness (0.5 μm)
solid core (1.7 μm)
product line
BIOshell
feature
endcapped: no
analyte chemical class(es)
glycans
manufacturer/tradename
BIOshell
packaging
1 ea of
parameter
1000 bar max. pressure (14500 psi)
1000 bar pressure (14500 psi)
60 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × I.D.
15 cm × 2.1 mm
surface area
135 m2/g
matrix
spherical silica particle platform
superficially porous particle
matrix active group
hydroxy, penta- phase
particle size
2.7 μm
pore size
90 Å
operating pH range
2-9
separation technique
hydrophilic interaction (HILIC)
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General description
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Articles
The BIOshell™ Glycan column is ideal for the separation and quantitation of isolated glycans using the HILIC method for accurate glycoprotein analysis.
The use of PNGase Fast denaturing buffer and enzyme yielded results similar to a conventional 20-hour protocol with overnight digest while reducing workflow time to about 1 hour with a 15-minute digest.
Step-by-step workflows for the intact mass analysis, peptide mapping, and N-glycan analysis of the monoclonal antibody― adalimumab, for an accurate characterization of the critical quality attributes (CQAs) to ensure drug safety and efficacy. Read more.
Protocols
Separation of Dextran Ladder, Glycan standard for HPLC
A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.
Related Content
HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.
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