51614-U
Ascentis® Express Phenyl-Hexyl, 2 μm UHPLC Column
2 μm particle size, L × I.D. 5 cm × 3 mm
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About This Item
UNSPSC Code:
41115700
NACRES:
SB.52
material
stainless steel column
Quality Level
agency
suitable for USP L11
product line
Ascentis®
feature
endcapped
manufacturer/tradename
Ascentis®
packaging
1 ea of
parameter
1000 bar max. pressure (14500 psi)
60 °C temp. range
technique(s)
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × I.D.
5 cm × 3 mm
surface area
120 m2/g
impurities
<5 ppm (metals)
matrix
Fused-Core particle platform
superficially porous particle
matrix active group
dimethylphenylhexyl phase
particle size
2 μm
pore size
90 Å pore size
pH
2-9
application(s)
food and beverages
separation technique
reversed phase
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General description
Ascentis® Express 2μm Phenyl-Hexyl UHPLC columns are based on a 90 Å Fused-Core® particle design.The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4-micron thick porous shell and the small overall particle size of 2-microns. The bonded, endcapped, dimethylphenylhexyl stationary phase of Ascentis Express 2μm Phenyl-Hexyl provides a stable, reversed phase packing with enhanced p-p and mild steric interactions due to the relatively long-chain hexyl linkage to the base silica. Ascentis Express 2μm Phenyl-Hexyl can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
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C M Chavez-Eng et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1011, 204-214 (2016-01-17)
An ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of (4S,5R)-5-[3,5-bis (trifluoromethyl)phenyl]-3-{[4'-fluoro-5'-isopropyl-2'-methoxy-4-(trifluoromethyl)biphenyl-2-yl] methyl}-4-methyl-1,3-oxazolidin-2-one (anacetrapib, I) and [(13)C5(15)N]-anacetrapib, II in human plasma has been developed to support a clinical study to determine the absolute bioavailability of I.
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
Petr Chocholouš et al.
Talanta, 103, 221-227 (2012-12-04)
Currently, for Sequential Injection Chromatography (SIC), only reversed phase C18 columns have been used for chromatographic separations. This article presents the first use of three different stationary phases: three core-shell particle-packed reversed phase columns in flow systems. The aim of
Ivona Lhotská et al.
Analytical and bioanalytical chemistry, 408(12), 3319-3329 (2016-03-20)
A new fast and sensitive method based on on-line solid-phase extraction on a fused-core precolumn coupled to liquid chromatography with fluorescence detection has been developed for ochratoxin A (OTA) and citrinin (CIT) determination in lager beer samples. Direct injection of
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
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