Skip to Content

All Photos(1)

Documents

53922-U

Ascentis^® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 4.6 mm

Sign Into View Organizational & Contract Pricing

About This Item

UNSPSC Code:

41115700

eCl@ss:

32110501

NACRES:

SB.52

Recommended Products

Slide 1 of 9

1 of 9

Supelco

53917-U

Ascentis^® Express RP-Amide, 2.7 μm HPLC Column

Supelco

53931-U

Ascentis^® Express RP-Amide, 2.7 μm HPLC Column

Supelco

53912-U

Ascentis^® Express RP-Amide, 2.7 μm HPLC Column

Supelco

53913-U

Ascentis^® Express RP-Amide, 2.7 μm HPLC Column

Supelco

53911-U

Ascentis^® Express RP-Amide, 2.7 μm HPLC Column

Supelco

2637505

Inlet Liner, Direct (SPME) Type, Straight Design (unpacked)

Supelco

22988

Stainless Steel HPLC Fittings

Supelco

2633901

Inlet Liner, Direct (SPME) Type, Straight Design (unpacked)

Supelco

53838-U

Ascentis^® Express C8, 2.7 μm HPLC Column

material

stainless steel column

Quality Level

agency

suitable for USP L60

product line

Ascentis^®

feature

endcapped

manufacturer/tradename

Ascentis^®

packaging

1 ea of

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

5 cm × 4.6 mm

surface area

135 m²/g

impurities

<5 ppm metals

matrix

Fused-Core^® particle platform
superficially porous particle

matrix active group

amide, alkyl phase

particle size

2.7 μm

pore size

90 Å

operating pH range

2-9

application(s)

food and beverages

separation technique

reversed phase

Looking for similar products? Visit Product Comparison Guide

Related Categories

HPLC Columns & Accessories

Application

UHPLC Analysis of Sunscreens on Ascentis^® Express RP-Amide, 2.7 μm

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Application

Product No.

Description

Pricing

G005681

HPLC Analysis of Bisphenol A, Dimethylbisphenol A and Nonylphenol on Ascentis^® Express RP-Amide (UV Detection), application for HPLC

G005689

LC/MS Analysis of Conjugated Bile Acids on Ascentis^® Express RP-Amide, application for HPLC

guard cartridge

Product No.

Description

Pricing

53519-U

Ascentis^® Express RP-Amide, 2.7 μm Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 4.6 mm, pkg of 3 ea

related product

Product No.

Description

Pricing

14274

Acetonitrile with 0.1% ammonium acetate, tested for UHPLC-MS

1.03726

Methanol, for UHPLC-MS LiChrosolv^®

1.03728

Water, for UHPLC-MS LiChrosolv^®

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Simultaneous and confirmative detection of multi-residues of β₂-agonists and β-blockers in urine using LC-MS/MS/MS coupled with β-receptor molecular imprinted polymer SPE clean-up.

S Fan et al.

Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 30(12), 2093-2101 (2013-10-22)

A liquid chromatography-linear ion-trap spectrometry (LC-MS³) method using β-receptor molecular-imprinted polymer (MIP) solid-phase extraction (SPE) as clean-up was developed to determine simultaneously and confirmatively residues of 25 β₂-agonists and 21 β-blockers in urine samples. Urine samples were subjected to enzymatic

A validated HPLC-MS/MS assay for quantifying unstable pharmacologically active metabolites of clopidogrel in human plasma: application to a clinical pharmacokinetic study.

Michael T Furlong et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 926, 36-41 (2013-04-02)

Clopidogrel is prescribed for the treatment of Acute Coronary Syndrome and recent myocardial infarction, recent stroke, or established peripheral arterial disease. A sensitive and reliable high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay was developed and validated to enable reliable

Simultaneous quantitative analyses of indole and oxindole alkaloids of Uncaria Hook in rat plasma and brain after oral administration of the traditional Japanese medicine Yokukansan using high-performance liquid chromatography with tandem mass spectrometry.

Hirotaka Kushida et al.

Biomedical chromatography : BMC, 27(12), 1647-1656 (2013-07-03)

Uncaria Hook (UH) alkaloids are involved in the beneficial effects of Yokukansan. However, the pharmacokinetics of UH alkaloids after oral administration of Yokukansan has not yet been sufficiently investigated. Therefore, we developed and validated a sensitive and specific high-performance liquid

Simultaneous detection of residues of 25 β₂-agonists and 23 β-blockers in animal foods by high-performance liquid chromatography coupled with linear ion trap mass spectrometry.

Fan Sai et al.

Journal of agricultural and food chemistry, 60(8), 1898-1905 (2012-02-15)

A sensitive method has been developed for the simultaneous determination of residues of 25 β₂-agonists and 23 β-blockers in animal foods by high-performance liquid chromatography coupled with linear ion trap mass spectrometry (HPLC-LIT-MS). This method is based on a new

Comparative study of new shell-type, sub-2 micron fully porous and monolith stationary phases, focusing on mass-transfer resistance.

Erzsébet Oláh et al.

Journal of chromatography. A, 1217(23), 3642-3653 (2010-04-23)

Today sub-2 microm packed columns are very popular to conduct fast chromatographic separations. The mass-transfer resistance depends on the particle size but some practical limits exist not to reach the theoretically expected plate height and mass-transfer resistance. Another approach applies

View All Related Papers

Articles

Ascentis® Express RP-Amide HPLC Columns

Sigma-Aldrich.com presents an article about innovative particle technology and RP-Amide bonding chemistry provide the benefits of speed, resolution, selectivity, aqueous stability, and improved peak shape for bases.

Synthetic Cannabinoid Analysis

A large variety of cannabinoid drug standards, along with an HPLC method with the Supelco Ascentis® Express RP-Amide column, are presented in this article.

Ascentis® Express RP-Amide Columns for HPLC and UHPLC

Improve your separation of basic compounds with our Ascentis® Express RP-Amide HPLC columns from the Supelco® portfolio.

Protocols

HPLC Analysis of Bisphenol A, Dimethylbisphenol A and Nonylphenol on Ascentis® Express RP-Amide (UV Detection)

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle related compounds

Chromatograms

LC/MS Analysis of Conjugated Bile Acids on Ascentis^® Express RP-Amide

application for HPLC

HPLC Analysis of Bisphenol A, Dimethylbisphenol A and Nonylphenol on Ascentis^® Express RP-Amide (UV Detection)

application for HPLC

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service