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582713-U

Supelco

BIOshell IgG 1000 Å C18, 2.7 μm HPLC Column

column L × I.D. 10 cm × 4.6 mm

Synonym(s):

BIOshell IgG 1000 Å C18, 2.7 μm HPLC Column

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About This Item

UNSPSC Code:
41115700

material

stainless steel column

agency

suitable for USP L1

product line

BIOshell

feature

endcapped

packaging

1 ea of

extent of labeling

1.4% Carbon loading

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

column L × I.D.

10 cm × 4.6 mm

surface area

22 m2/g

matrix

Fused-Core® particle platform
superficially porous particle

matrix active group

C18 (diisobutyloctadecylsilane) bonding phase

particle size

2.7 μm

pore size

1000 Å pore size

operating pH range

1-8

separation technique

reversed phase

Related Categories

General description

BIOshell IgG 1000 Å C18 is a high-speed, high-performance liquid chromatography column based on a new wide-pore (1000 Å) Fused-Core® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5 μm thin porous shell and the small overall particle size of 2.7 μm. The sterically protected C18 bonded phase of BIOshell IgG 1000 Å C18 provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds, such as proteins.

Application

UHPLC Analysis of Reduced IgG1 on BIOshell IgG 1000 Å C18
UHPLC Analysis of Proteins on BIOshell IgG 1000 Å C18, 2.7 μm
UHPLC Analysis of Proteins on BIOshell IgG 1000 Å C18, 2.7 μm
UHPLC Analysis of IgG2 on BIOshell IgG 1000 Å C4, C18, and Diphenyl, 2.7 μm

Analysis Note

The target analytes include monoclonal antibodies, antibody-drug conjugates, antibody fragments and large proteins with molecular weights up to 500 kDa.

Legal Information

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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