58365
SUPELCOSIL™ LC-Si HPLC Column
5 μm particle size, L × I.D. 25 cm × 10 mm
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
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agency
suitable for USP L3
Quality Level
manufacturer/tradename
SUPELCOSIL™
parameter
≤70 °C temp. limit
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
25 cm × 10 mm
surface area
170 m2/g
matrix
silica gel, spherical particle platform
matrix active group
silica phase
particle size
5 μm
pore size
120 Å pore size
pH range
2-7.5
application(s)
food and beverages
separation technique
hydrophilic interaction (HILIC)
normal phase
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General description
A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent. Non-polar compounds will elute from the column first, while polar solutes show stronger interaction with the silanol groups on the silica surface. The polar selectivity of silica is very helpful for separating mycotoxins. Silica is frequently used in preparative chromatography due to its low operating cost and back pressure compared to reversed-phase columns. Silica is particularly popular among organic chemists as a low-pressure preparative tool. The unmodified silica in SUPELCOSIL LC-Si columns also separate positional isomers.
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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J J Agren et al.
Lipids, 37(6), 613-619 (2002-07-18)
Normal-phase HPLC resolution of sn-1,2(2,3)- and x-1,3-DAG generated by partial Grignard degradation from natural TAG was carried out with both (R)-(-) and (S)-(+)-1-(1-naphthyl)ethylurethane derivatives. The diastereomeric sn-1,2- and sn-2,3-DAG derivatives were resolved using two Supelcosil LC-Si (5 microm, 25 cm
Mohammed Shahid Ali et al.
Journal of pharmaceutical and biomedical analysis, 43(1), 158-167 (2006-08-05)
A new HILIC method has been developed for the simultaneous determination of pseudoephedrine hydrochloride (PSH), diphenhydramine hydrochloride (DPH) and dextromethorphan hydrobromide (DXH) in cough-cold syrup. Mobile phase consists of methanol:water (containing 6.0 g of ammonium acetate and 10 mL of
R Lovlin et al.
Journal of chromatography. B, Biomedical applications, 679(1-2), 196-198 (1996-04-26)
The stereospecific HPLC assays reported for ketoprofen (KT) mainly utilize indirect approaches. These assays involve the formation of amide diastereomeric derivatives, which are then separated by chromatography. The advantages of indirect methods include versatility, good sensitivity and cost effectiveness; however
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