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584610-U

Supelco

Ascentis® Express 160 Å Phenyl-Hexyl, 2.7 μm HPLC Column

column L × I.D. 10 cm × 2.1 mm

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About This Item

UNSPSC Code:
41115700

material

stainless steel column

agency

suitable for USP L11

product line

Ascentis®

feature

endcapped

packaging

1 ea of

extent of labeling

4.7% Carbon loading

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

column L × I.D.

10 cm × 2.1 mm

surface area

90 m2/g

matrix

Fused-Core® particle platform
superficially porous particle

matrix active group

Phenyl-Hexyl (dimethylphenyl-hexylsilane) bonding phase

particle size

2.7 μm

pore size

160 Å pore size

operating pH range

2-9

separation technique

reversed phase

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General description

Ascentis® Express 160 Å Phenyl-Hexyl is a high-speed, high-performance liquid chromatography column based on a 160Å Fused-Core® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency for high molecular weight solutes (up to 20 kDa) due to the shallow diffusion paths in the 0.5 μm thick porous shell and the small overall particle size of 2.7 μm. The endcapped, phenyl-hexyl bonded phase provides a stable, reversed phase packing with a pore structure and pore size that is optimized for reversed-phase HPLC separations of peptides and polypeptides, using typical acidic mobile phases favored for proteomic applications.

Analysis Note

Target analytes include electron-poor molecules, aromatic or unsaturated compounds (ketones, nitriles, alkenes), as well as peptides and polypeptides < 20 kDa.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Certificates of Analysis (COA)

Lot/Batch Number
USEHP001279
USEHP001274

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