59132
SUPELCOSIL™ LC-NH2-NP HPLC Column
5 μm particle size, L × I.D. 25 cm × 4.6 mm
Synonym(s):
SUPELCOSIL™ LC-NH2-NP 5UM 25CMX4.6MM HPLC COLUMN
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
Recommended Products
agency
suitable for USP L8
Quality Level
feature
endcapped
manufacturer/tradename
SUPELCOSIL™
extent of labeling
3% Carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
25 cm × 4.6 mm
surface area
170 m2/g
surface coverage
surface coverage 5.1 μmol/m2
matrix
silica gel, spherical particle platform
matrix active group
amino phase
particle size
5 μm
pore size
120 Å
pH
2-7.5
application(s)
food and beverages
separation technique
normal phase
Related Categories
General description
An amino phase dedicated to normal-phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal-phase chromatography. Normal-phase chromatography is especially useful when the analytes are not water soluble -- for example, the fat-soluble vitamins A, D, E, and K.
These columns should be used with non-aqueous mobile phases only.
These columns should be used with non-aqueous mobile phases only.
SUPELCOSIL LC-NH2-NP is an amino phase dedicated to normal phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal phase chromatography.
Features and Benefits
• show stable retention in normal-phase separations
• are less sensitive to small or varying amounts of water in mobile phases, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins
• are less sensitive to small or varying amounts of water in mobile phases, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins
SUPELCOSIL LC-NH2-NP columns:
• show stable retention in normal phase separations
• are less sensitive to small or varying amounts of water in the mobile phase, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins
• show stable retention in normal phase separations
• are less sensitive to small or varying amounts of water in the mobile phase, relative to unmodified silica
• provide excellent separations of fat-soluble vitamins
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Sainath Babu et al.
Toxicology mechanisms and methods, 25(5), 410-416 (2015-05-30)
We have developed a simple, reversed-phase high-performance liquid chromatography (RP-HPLC) method for the determination of bisphenol A (BPA) in thermal paper cash register receipts (CRs). The method is suitable for analysis of other types of bisphenols and it involves an
K K Peh et al.
International journal of pharmaceutical compounding, 4(3), 229-231 (2000-05-01)
A simple and selective high-performance liquid chromatography (HPLC) method using ultraviolet detection was developed for simultaneous determination of fusidic acid and betamethasone dipropionate in a cream formulation. A Supelcosil LC18 column was used for chromatographic separation. The mobile phase consisted
Vijay V Upreti et al.
Biomedical chromatography : BMC, 17(6), 385-390 (2003-09-19)
A simple and sensitive high-performance liquid chromatography (HPLC) method has been developed and validated for the determination of DRF-1042, a novel orally active camptothecin (CPT) analog, in human plasma. The sample preparation was a simple deproteinization with acidified methanol yielding
E M Koves
Journal of chromatography. A, 692(1-2), 103-119 (1995-02-10)
A comprehensive approach to the analysis for many drugs in postmortem blood and biological fluids using high-performance liquid chromatography and diode array detection has been developed. To reduce the likelihood of co-eluting interference components of postmortem blood or other drugs
S Djabarouti et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 799(1), 165-172 (2003-12-09)
The aim of this study was to develop a specific and sensitive high-performance liquid chromatographic (HPLC) assay for the determination of levofloxacin in human plasma, bronchoalveolar lavage and bone tissues. The sample extraction was based on a fully automated liquid-solid
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service