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BIOshell A400 Protein C18 (3.4 μm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

product name

BIOshell A400 Protein C18 Column, L × I.D. 10 cm × 2.1 mm, 3.4 μm particle size

material

stainless steel hardware (column)

Quality Level

agency

suitable for USP L1

description

Shell Thickness (0.2 μm)
Solid Core (3.0 μm)

product line

BIOshell

feature

endcapped

analyte chemical class(es)

proteins

manufacturer/tradename

BIOshell

packaging

1 ea of

extent of labeling

1% carbon loading

parameter

600 bar max. pressure
90 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

10 cm × 2.1 mm

surface area

~15 m2/g

matrix

spherical silica particle platform
superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

3.4 μm

pore size

3.4 μm particle size
400 Å

operating pH

1-8

separation technique

reversed phase

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General description

BIOshell 400 Å Protein C18 columns contain 3.4 μm advanced Fused-Core® particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns offer maximum hydrophobicity for resolving tricky hydrophilic and PEGylated proteins. The 3.4 μm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 μm, 300 Å fully porous C18 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 1 to 9.

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Legal Information

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Protocols

Separation of HPLC protein standard mixture, analytical standard

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