796-0657
HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1)
application for HPLC
About This Item
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description
Separated on Discovery® BIO PolyMA-WAX
technique(s)
HPLC: suitable
test parameters
column: Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)
mobile phase: [A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear)
flow rate: 0.5 mL/min
detector: UV, 280 nm
injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
suitability
application for HPLC
application(s)
life science and biopharma
Analysis Note
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