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808149

TSKgel® ODS-80Tm (5 µm) HPLC Columns

L x I.D. 25 cm × 4.6 mm, C18 (octadecyl) phase

Synonym(s):

TSKgel® Reversed Phase HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

product name

TSKgel® ODS-80Tm HPLC Column, phase C18 (octadecyl), L × I.D. 25 cm × 4.6 mm, 5 μm particle size

material

stainless steel

agency

suitable for USP L1

product line

TSKgel®

manufacturer/tradename

Tosoh ODS-80Tm, 08149

extent of labeling

15% Carbon, monolayer, endcapped

parameter

0-100% organic solvent
0-45 °C temperature
1.2 mL/min flow rate
310 bar max. pressure

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

425 m2/g

matrix

silica particle platform

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

100 Å pore diameter

operating pH

2.0-7.5

separation technique

reversed phase

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General description

TSKgel ODS-80Tm is a general purpose column for the analysis of low MW pharmaceuticals, bases, nucleosides and nucleotides. Common applications include purity checks and peptide mapping. Bonded phase pore size is indicated by the number in the product description, in this case TSKgel ODS-80Tm has 80Å nominal pore size. The pore size of the base silica is 100Å.

Application

TSKgel® ODS-80Tm HPLC Column may be used for the separation of:
  • Dehydroacetic acid, benzoic acid, sorbic acid and salicylic acid in cosmetic products during their determination by solid-phase extraction (SPE) followed by analysis using high performance liquid chromatography (HPLC) with ultraviolet (UV) detection.
  • Cilostazol and its metabolites in human urine during their determination by liquid-liquid extraction (LLE) followed by analysis using HPLC-UV.
  • Heterocyclic amines in food products during their analysis by LC coupled to mass spectrometry with electrospray ionization (ESI-MS), LC combined with tandem mass spectrometry (MS/MS) with ESI, LC coupled to atmospheric pressure chemical ionization (APCI)-MS/MS, LC with UV-diode array detection (DAD), LC with UV-fluorescence detection (FLD) and LC in conjunction with electrochemical detection, etc.
  • Rabeprazole and its metabolite, rabeprazole thioether in human plasma samples during their analysis by column switching HPLC with UV detection.
  • β-lactam antibiotic, cefaclor during its determination by HPLC-FLD.

Physical form

Shipped in 70% methanol - 30% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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Sensitive determination of a β-lactam antibiotic, cefaclor by liquid chromatography with chemiluminescence detection.
Kai M, et al.
Journal of Pharmaceutical and Biomedical Analysis, 30(6), 1765-1771 (2003)
Analysis of heterocyclic amines in food products: interlaboratory studies
Santos FJ, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 802(1), 69-78 (2004)
Determination of cilostazol and its metabolites in human urine by high performance liquid chromatography.
Tata PNV, et al.
Journal of Pharmaceutical and Biomedical Analysis, 24(3), 381-389 (2001)
Determination of rabeprazole and its active metabolite, rabeprazole thioether in human plasma by column-switching high-performance liquid chromatography and its application to pharmacokinetic study.
Uno T, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 824(1-2), 238-243 (2005)
E Mikami et al.
Journal of pharmaceutical and biomedical analysis, 28(2), 261-267 (2002-04-04)
A high-performance liquid chromatographic (HPLC) method for simultaneous determination of dehydroacetic acid (DHA), benzoic acid (BA), sorbic acid (SOA) and salicylic acid (SA) was developed for application to cosmetic products. Isocratic reversed-phase HPLC was employed for quantitative analysis using tetra-n-butylammonium

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